Roux carried out his experiment by plunging a hot needle into one of the
first two blastomeres, so that it is injured to such an extent that its
development is prevented. The same needle, without heating again, was
used for one or two other eggs, for, if the needle had been so hot in
the first instance that both blastomeres had been injured by the heat,
this might not happen in the second or the third egg. It was found that
amongst the eggs that had been operated upon in this way, some had been
so much injured that neither blastomere developed, others had been so
little injured that both blastomeres developed, but in the successful
operations the uninjured blastomere developed, while the injured one did
not. In the last case the uninjured blastomere divided, and produced a
large number of cells. A segmentation cavity was present in the upper
part of the hemisphere (Fig. 61, _A_). The injured half remained in
contact with the other, completing the sphere, but it did not segment. A
half-embryo developed from the uninjured half, as shown in Fig. 61, _B_,
_C_. This embryo has a half-medullary fold along the side in contact
with the injured half. At the anterior end somewhat more than half a
head is present, and at the posterior end there is a half-blastopore.
The cross-sections[109] (Fig. 61, _C_), through the embryo, show that
beneath the half-medullary fold a rod-like notochord is present, which
is made up apparently of fewer cells than the normal notochord, but it
has, in cross-section, a round and not a half form. At the side, the
mesoderm is present, as in the normal embryo, and it has produced the
characteristic mesoblastic somites. An archenteron is formed in the
half-embryo, and, since it is smaller than the normal, it may, perhaps,
be called a half-archenteron. The embryo is, therefore, in most respects
a half-structure. The head is, however, nearly a whole head, but whether
this is due to a whole head developing out of material derived entirely
from one of the two blastomeres, or whether, as Roux supposes, a portion
of the material of the injured blastomere has been worked over, _i.e._
“post-generated,” remains, I think, an open question.
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