The uninjured blastomere first gives rise to a half-embryo. After this
has been accomplished, other changes take place that “reorganize,”
according to Roux, the material of the injured half in such a way that
the missing half of the embryo is formed by a process that Roux calls
“post-generation.” This process can be studied only by means of
sectioning the embryos, and since the eggs may be injured to a varying
extent, there must be some uncertainty in making out the sequence of
events. It is found that the yolk of the injured blastomere is
vacuolated in places, and that the protoplasm in the path of the needle
has been killed (Fig. 61, _A_). Irregular pieces of chromatin are found
in the protoplasm, which seem to come from an irregular breaking up of
the nucleus.
The changes that lead to the reorganization of the injured half may take
place at different times in different eggs. Roux describes three kinds
of reorganization phenomena. The first includes the formation of new
cells in the injured half. Nuclei, surrounded by finely granular
protoplasm, appear in the protoplasm of the injured blastomere. These
nuclei arise from two sources: in part from the scattered chromatin of
the injured blastomere itself, and in part from nuclei, or from cells
without walls that have emigrated from the developing half. Around these
nuclei, as centres, the protoplasm (with its contained yolk) of the
injured half breaks up into cells. This cellulation of the yolk may take
place in different eggs at different times. In some cases it may not
have appeared as late as the gastrula stage of the uninjured half; in
others, it may take place at the time when the uninjured half is
segmenting.[110] The formation of the cells in the injured half begins
always near the developing half, and extends thence into the injured
parts. The new cells are of different sizes, but are larger than those
of the uninjured half.
The cellulation of the yolk takes place only in the least injured parts
of the protoplasm. Where the protoplasm and yolk have been much injured,
they are changed over by the second method of reorganization. This part
of the blastomere is either actually devoured by wandering cells, or is
slowly changed under the influence of the neighboring cells, so that it
becomes a part of these cells.
The surface of the injured half is covered over by ectoderm that grows
directly from the developing half (third method of reorganization),--at
least this happens where the protoplasm has been much injured. In other
parts of the injured half the new cells that have appeared in this part,
and that lie at the surface, become new ectoderm.
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