Research methods in ecologyClements, Frederic E. (Frederic Edward)
Science
Research methods in ecology
Clements, Frederic E. (Frederic Edward)
Plant ecology; Plant ecology -- Methodology
Hesselmann[14], in his study of open woodlands in Sweden, has employed a
method essentially similar to the preceding. Young plants of various
species were transferred to pots in the field, where they were allowed
to grow for several months before a series of weighings was made to
determine the amount of transpiration. Since weighing is the measure
used in each, both methods are equally accurate. The one has a certain
advantage in that the pots are, perhaps, more easily handled, while the
other has the advantage of maintaining the normal relation of soil and
roots, a condition more or less impossible in a pot. In both instances
the weighing should be done in the habitat, which was not the case in
Hesselmann’s researches.
The slight value of the potometer, which has had a vogue far beyond its
merits, is indicated by the following table. These results were obtained
from three plants of _Helianthus annuus_; III was left undisturbed in
the pot where it had been growing, IV was placed in a potometer, after
the root had been cut off, and V was an entire plant placed in a
potometer. The amount of transpiration is indicated in grams per square
decimeter of leaf surface. The plants were kept in diffuse light, except
for a period of two hours (8:00 to 10:00 A.M.) on the last day, when
they were in full sunshine at a temperature of 75° F. Plant IV wilted so
promptly in the sunshine that it was found necessary to conclude the
experiment in diffuse light.
═════╤═════╤═════╤═════╤═════╤═════╤═════╤═════╤═════╤═════╤═════╤═════
│ 8 │ 5 │ 8 │ 5 │ 8 │ 5 │ 8 │ 10 │ 5 │ 8 │Total
│A.M. │P.M. │A.M. │P.M. │A.M. │P.M. │A.M. │A.M. │P.M. │A.M. │
─────┼─────┼─────┼─────┼─────┼─────┼─────┼─────┼─────┼─────┼─────┼─────
III│ 2.9 │ 7.3 │ 2.4 │ 6.0 │ 1.7 │ 1.6 │ 2.0 │ 3.4 │ 2.0 │ 1.8 │31.1
IV│ 4.7 │ 7.2 │ 2.9 │ 2.3 │ 1.0 │ 0.6 │ 0.9 │ 0.5 │ 0.5 │ 0.4 │21.0
V│ 3.7 │ 5.3 │ 3.2 │ 4.8 │ 2.5 │ 1.6 │ 3.0 │ 2.6 │ 1.6 │ 2.6 │30.9
─────┴─────┴─────┴─────┴─────┴─────┴─────┴─────┴─────┴─────┴─────┴─────
The cut plant, IV, lost more water the first day than either of the
others, but the water loss soon decreased, and at the end of the period
was almost nil. The total transpiration for III and V is much the same,
but the range of variation for periods of 12 hours is from +2 to –1
gram. This experiment is taken as a fair warrant that the use of cut
stems in potometers can not give accurate results. It is inconclusive,
however, as to the merits of potometric values obtained by means of the
entire plant, and further studies are now being made with reference to
this point.
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