Research methods in ecologyClements, Frederic E. (Frederic Edward)
Science
Research methods in ecology
Clements, Frederic E. (Frederic Edward)
Plant ecology; Plant ecology -- Methodology
=51. Terms.= The terms, physiological water-content, and physical
water-content, are awkward and not altogether clear in their
application. It is here proposed to replace them by short words which
will refer directly to the availability of the soil water for absorption
by the plant. Accordingly, the total amount of water in the soil is
divided into the available and the non-available water-content. The
terms suggested for these are respectively, _holard_ (ὅλos, whole,
ἅpδov, water), _chresard_ (χοῆςις, use), and _echard_ (ἕχω, to
withhold).
=52. Chresard determinations under control.= The determination of the
chresard in the field is attended with peculiar difficulties. In
consequence, the method of obtaining it under control will first be
described. The inquiry may be made with reference to soils in general or
to the soil of a particular formation. In the last case, if the plants
used are from the same formation, the results will have almost the value
of a field determination. When no definite habitat is the subject of
investigation, an actual soil, and not an artificial mixture, should be
used, and the plants employed should be mesophytes. The individual
plants are grown from seeds in the proper soils, and are repotted
sufficiently often to keep the roots away from the surface. The last
transfer is made to a pot large enough to permit the plant to become
full-grown without crowding the roots. The pot should be glazed inside
and out in order to prevent the escape of moisture. This interferes
slightly with the aeration of the soil, but it will not cause any real
difficulty. The plant is watered in such a way as to make the growth as
normal as possible. After it has become well established, three soil
samples are taken in such a manner that they will give the variation in
different parts of the pot. One is taken near the plant, the second
midway between the plant and the edge of the pot, and the third near the
edge. The depth is determined by the size of the pot and the position of
the roots. The holard is determined for these in the usual way, but the
result is expressed with reference to 100 grams of dry soil; the average
is taken as representative. The soil is then allowed to dry out slowly,
as sudden drouth will sometimes impair the power of absorption and a
plant will wilt although considerable available water remains. Plants
often wilt in the field daily for several successive hot dry days, and
become completely turgid again during the night. If the drying out takes
place slowly, the plant will not recover after it has once begun to
wilt. The proper time to make the second reading is indicated by the
pronounced wilting of the leaves and shoots. Complete wilting occurs, as
a rule, only after the younger parts have drawn for some time upon the
watery tissues of the stem and root, by which time evaporation has
considerably deceased the water in the soil. It is a well-known fact
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