Research methods in ecologyClements, Frederic E. (Frederic Edward)
Science
Research methods in ecology
Clements, Frederic E. (Frederic Edward)
Plant ecology; Plant ecology -- Methodology
it has been found practicable to obtain leaf prints by exposing the leaf
in a printing frame, upon solio paper, at the same time that the
epidermis print is made. In a few species both the upper and lower
epidermis can be removed and the amount of light absorbed determined
directly by exposing the strip covered with the chlorenchym. Generally,
however, this must be computed by subtracting the sum of the per cents
of reflected and transmitted light from 100 per cent, which represents
the total light.
[Illustration: Fig. 15. Leaf print: exposure as before. Sun and shade
leaves of _Achillea lanulosa_, _Capnoides aureum_, _Antennaria
umbrinella_, _Galium boreale_, and _Potentilla propinqua_.]
=90. Leaf and epidermis prints.= In diphotic leaves the screening effect
of the lower epidermis may be ignored. Isophotic sun leaves, i. e.,
those nearly upright in position or found above light-colored,
reflecting soils, are usually strongly illuminated on both sides, and
the absorbed light can be obtained only by measuring the screening
effect of both epiderms. Shade leaves and submerged leaves often contain
chloroplasts in the epidermis, and the above method can not be applied
to them. In fact, in habitats where the light is quite diffuse,
practically all incident light is absorbed. The rare exceptions are
those shade leaves with a distinct bloom. In addition to their use in
obtaining the amount of light absorbed, both leaf and epidermis prints
are extremely interesting for the direct comparison of light relations
in the leaves of species belonging to different habitats. The relative
screening value of the upper and lower epidermis, or of the
corresponding epiderms of two ecads or two species, is readily
ascertained by exposing the two side by side in sunshine, over the slit
in the photometer. For leaf prints fresh leaves are desirable, though
nearly the same results can be obtained from leaves dried under
pressure. The leaves are grouped as desired on the glass of a printing
frame, and covered with a sheet of solio. They are then exposed to full
sunlight, preferably at meridian, and the prints evaluated by means of
the multiple standard. This method is especially useful in the
comparison of ecads of one species. These differences due to transmitted
light are very graphic, and can easily be preserved by “toning” the
print in the usual way.
_Expression of Results_
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