(1) Operating in this way upon brewers' grains the furfuroid was obtainable as the chief constituent of a solution for which the following experimental numbers were determined:--Total dissolved solids, 28.0 p.ct. of original 'grains'; furfural, 39.5 p.ct. of total dissolved solids, as compared with 12.5 p.ct. of total original grains; cupric reduction (calc. to total solids), 110 (dextrose = 100) osazone; yield in 3 p.ct. solution, 35 p.ct. of weight of total solids. Pentosazone Analysis N 17.1 17.3 17.07 C 62.5 62.3 62.2 H 6.4 6.5 6.1 Melting-point 146 deg.-153 deg. From these numbers it is seen that of the total furfuroids of the original 'grains' 84 p.ct. are thus obtained in solution in the fully hydrolysed form, which is that of a pentose or pentose derivative. It was, however, found impossible to obtain any crystallisation from the neutralised (BaCO_{3}) and concentrated solution, the syrup being kept for some weeks in a desiccator. It was noted at the same time that the colour reaction of the original solution with phloroglucol and hydrochloric acid was a deep violet, in contradistinction to the characteristic red of the pentoses. On oxidation with hydrogen peroxide, in the proportion of 1 mol. H_{2}O_{2} to 1 mol. of the carbohydrate in solution, carbonic anhydride was formed in quantity = 20.0 p.ct. of the latter. Fermentation (yeast) experiments also showed a divergence from the resistant behaviour of the pentoses, a considerable proportion of the furfuroid disappearing in a normal fermentation. (2) The quantitative methods above described were employed in investigating the barley plant at different stages of its growth. The green plant was extracted with alcohol, the residue freed from alcohol and subjected to acid hydrolysis. The hydrolysed extract was neutralised and fermented. In the early stages of growth the furfuroids were completely fermented, i.e. disappeared in the fermentation. In the later stages this proportion fell to 50 p.ct. In the earlier stages, moreover, the normal hexose constituents of the permanent tissue were hydrolysed in large proportion by the acid, whereas in the matured straw the hydrolysis is chiefly confined to the furfuroids. In the early stages also the permanent tissue yields an extract with relatively low cupric reduction, showing that the carbohydrates are dissolved by the acid in a more complex molecular condition. These observations confirm the view that the furfuroids take origin in a hexose-pentose series of transformations. The proportion of furfuroid groups to total carbohydrates varies but little, viz. from 1/3 in the early stages to a maximum of 1/4 at the flowering period. At this period the differentiation of the groups begins to be marked.