The nitrogen found in the urine can be expressed as albumen by multiplying it by 6.25 (Neumeister), at the same time making a correction for the nitrogen in the fæces as described above. This is all that there is to say about the analysis of the urine, which is of so much importance for our experiments. In order to show the practical application, I will add the following analyses, as actually made in exercising an influence over sex to obtain male offspring. * * * * * 1. Case of a woman twenty-three years old, who, before anything was done to influence the sex of her offspring, had been married five years, and had given birth to two girls. The urine was collected from eight in the morning until the same hour of the next day in a measuring-glass. The quantity in twenty-four hours was 1,650 cubic centimetres. Analysis gave the following results:-- ANALYSIS. _Reaction_: Acid. _Specific gravity_: 1017. _Quantity in 24 hours_: 1650. _Color_: Light yellow, fairly pale. _Sugar_: None perceptible with Trommer’s test, the fermentation test, and Nylander’s test. _Phenylhydrazin test_: Negative. _Optical rotation_: Very slight, not determinable. _Reducing substances_: 0.135 per cent. _Nitrogen_: 12.76 (Correction 0.94). _Nitrogen as albumen_: 79.75. I recommended that more meat should be taken, and that sugar and other forms of carbo-hydrate should be avoided. After the lapse of eight days, I again procured urine for examination. Analysis gave the following results:-- ANALYSIS. _Reaction_: Acid. _Specific gravity_: 1018. _Quantity in 24 hours_: 1050. _Color_: Somewhat darker than on 17, 1. _Sugar_: None perceptible with Trommer’s test, the fermentation test, and Nylander’s test. _Phenylhydrazin test_: Negative. _Optical rotation_: Not determinable. _Reducing substances_: 0.15 per cent. _Nitrogen_: 13.5 (Correction: 0.94). _Nitrogen as albumen_: 84.37. The result was that the reducing substances and the nitrogen (expressed as albumen) had increased. The diet of this woman was constantly altered in the direction of increasing the amount of albumen, until, after the lapse of about three weeks, the following results were obtained:-- ANALYSIS. _Reaction_: Acid. _Specific gravity_: 1030. _Quantity in 24 hours_: 1000. _Color_: Dark, brownish-yellow. _Sugar_: Cannot be determined by Trommer’s test, the fermentation test, and Nylander’s test. _Phenylhydrazin test_: Positive; the glycosazon-crystals have melting-point 110° cent., therefore do not result from sugar. _Optical rotation_: 0.2 per cent. lævo-rotatory. _Reducing substances_: 0.32 per cent. _Nitrogen_: 21.9 grammes (Correction: 1 gramme). _Nitrogen as albumen_: 136.8 grammes.