1. That the disease favors the growth of these bacilli by affording them a suitable soil. If so, it would mean that the bacillus in question is most widely diffused, since it has been found in such different regions as Egypt, India, and France; whereas the contrary is the case, for the bacilli do not occur in other diseases, nor in the healthy, nor apart from human beings in localities most favorable to bacterial life. They only appear with the cholera. 2. It might be said that cholera produces conditions leading to a change in form and properties of the numerous intestinal bacteria, a pure hypothesis; the only instance of such a conversion refers to a change of physiological and pathogenic action, and not of form. Anthrax bacilli under certain conditions lose their pathogenic power, but undergo no change in shape; and that is an instance of a loss of pathogenic properties, while there is no analogy to support the view of the harmless intestinal bacteria becoming the deadly cholera bacilli. The more bacterial morphology is studied, the more certain it is that bacteria are constant in their form; moreover, the comma bacillus retains its special characters unchanged through many generations of culture. 3. Lastly, there is the view that the cholera process and the comma bacilli are intimately related, and there is no other conceivable relation but that the bacilli precede the disease and excite it. "For my own part," said Dr. Koch, "the matter is proved that the comma bacilli are the cause of cholera." Dr. Koch then described his attempts to inoculate lower animals with the bacillus, and explained the cause of his failure in the natural immunity of the animals against the disease.