It becomes then clearly manifest that these organisms, lowly and little as they are, arise in fertilized parental products. There is no more caprice in their mode of origin than in that of a crustacean or a bird. Their minuteness, enormous abundance, and universal distribution is the explanation of their rapid and practically ubiquitous appearance in a germinating and adult condition. The presence of putrefiable or putrescent matter determines at once the germination of the always-present spore. But a new question arises. These spores are definite products. In the face of some experimental facts one was tempted to inquire: Have these spores any capacity to resist heat greater than the adults? It was not easy to determine this question. But we at length were enabled to isolate the germs of seven separate forms, and by means of delicate apparatus, and some twelve months of research, to place each spore sac in an apparatus so constructed that it could be raised to successive temperatures, and without any change of conditions examined on the stage of the microscope. In this way we reached successive temperatures higher and higher until the death point--the point beyond which no subsequent germination ever occurred--was reached in regard to _each_ organism. The result was striking. The normal death point for the adult was 140° F. One of the monads emitted from its sac minute mobile specks--evidently living bodies--which rapidly grew. These we always destroyed at a temperature of 180° F. Three of the sacs emitted spores that germinated at every temperature under 250° F. Two more only had their power of germination destroyed at 260° F. And one, the least of all the monad forms, in a heat partially fluid and partially dry, at all points up to 300° F. But if wholly in fluid it was destroyed at the point of 290° F. The average being that the power of heat resistance in the spore was to that of the adult as 11 to 6. From this it is clear that we dare not infer spontaneous generation after heat until we know the life-history of the organism.