When sugar is present the bichromate would be reduced by the sugar, hence this method is not applicable. In this case remove the fatty acids as before, neutralize an aliquot portion with milk of lime, evaporate to 10 cubic centimeters, add 2 grams of sand and milk of lime containing about 2 grams of calcium hydroxide and evaporate almost to dryness. Treat the moist residue with 5 cubic centimeters of 96 per cent. alcohol, rub the whole mass into a paste, then constantly stirring, heat on a water bath and decant into a 250 cubic centimeter graduated flask. Repeat the washing with 5 cubic centimeters of alcohol five or six times, each time pouring the washings into the flask; cool the flask to room temperature and fill to the mark with 96 per cent. alcohol, agitate the flask until well mixed and filter through a dry filter paper. Take 200 cubic centimeters of the nitrate and evaporate to a syrupy consistency over a safety water bath. Wash the liquor into a stoppered flask with 20 cubic centimeters of absolute alcohol, add 30 cubic centimeters of absolute ether 10 cubic centimeters at a time, shaking well after each addition and let stand until clear. Pour off the solution through a filter into a weighed dish and wash out the flask with a mixture of three parts absolute ether and two parts absolute alcohol. Evaporate to a syrup, dry for one hour at the temperature of boiling water, weigh, ignite and weigh again. The loss is glycerine. This multiplied by 5/4 gives the total loss for the aliquot portion taken. The glycerine may also be determined by the acetin or bichromate methods after driving off the alcohol and ether if so desired. SUGAR IN SOAP. To determine sugar in soap, usually present in transparent soaps, decompose a soap solution of 5 grams of soap dissolved in 100 cubic centimeters of hot water with an excess of hydrochloric acid and separate the fatty acids as usual. Filter the acid solution into a graduated flask and make up to the mark. Take an aliquot containing approximately 1 per cent. of reducing sugar and determine the amount of sugar by the Soxhlet method.[18] GLYCERINE ANALYSIS. The methods of analyzing glycerine varied so greatly due to the fact that glycerine contained impurities which acted so much like glycerine as to introduce serious errors in the determinations of crude glycerine. This led to the appointment of committees in the United States and Europe to investigate the methods of glycerine analysis. An international committee met after their investigations and decided the acetin method should control the buying and selling of glycerine, but the more convenient bichromate method in a standardized form might be used in factory control and other technical purposes. The following are the methods of analysis and sampling as suggested by the international committee: SAMPLING.