It is not always, then, that the commonly recognized distinction between surface soil and subsoil based upon humus content can be maintained. But the observation of everything bearing upon this point is of the utmost importance in determining both the agricultural value and the mode of treatment of the land. _Losses of Humus from Cultivation and Fallowing._—The fact that humus accumulates in woodlands and meadows, where no cultivation is given, would naturally lead to the converse conclusion, viz., that cultivation causes loss of humus and of its constituents. That this is actually the case is recognized and widely acted upon in practice, and there is no question that the general acceptance of stable manure as the most widely useful fertilizer, despite its usually low content of plant-food ingredients, is based upon the fact that it supplies vegetable matter, in a condition highly favorable to its conversion into humus. The most direct and cogent proof of the depletion of the soil of both humus and nitrogen by continuous cultivation of cereal grains has been given by Snyder,[46] who determined the loss both of humus and of nitrogen suffered by a Minnesota soil during eight years’ continuous cultivation of wheat. The total loss of nitrogen was 1700 pounds per acre, while only 350 pounds were utilized by the crop; about 1400 pounds being dissipated as gas or leached out as nitrates. A conservative estimate of the loss of humus suffered during the same period was about a ton per acre annually, and this loss seriously decreased not only the nitrogen-content, but rendered the soil more compact and less retentive of moisture. But by rotation of the wheat with clover in alternate years, very nearly an equilibrium of both humus and nitrogen-content was obtained. In addition, the amount of available mineral plant-food was decreased by continuous grain culture. Ladd has made similar observations in North Dakota, with similar results. [46] Bull. No. 70 Minn. Exp’t Station, 1905. That excessive aeration results in serious losses of humus as well as of nitrogen, is very obvious in the arid region, where it is the habit to maintain on the surface of orchards and vineyards during the dry, hot summers, a thick mulch of well-tilled soil, thus preventing loss of water by evaporation. In the course of years this surface soil becomes so badly depleted of humus that good tilth becomes impossible, the soil becoming light-colored and compacted; while the loss of nitrogen is indicated by the small size of the orchard fruits. Similar losses are of course sustained in the practice of bare summer-fallow, which at one time was almost universal in portions of the arid region. The complete extirpation of weed growth thus brought about, at first considered an unmixed benefit, has ultimately had to be made up for by the practice of green-manuring; since in the arid region the use of stable manure encounters many difficulties.