ROOT-BACTERIA OR RHIZOBIA OF LEGUMES.—Among the most important bacteria, agriculturally, is that which enables plants of the leguminous order—(peas, beans, vetches, clovers, lupins, etc.),—to obtain their supply of nitrogen from the air independently of those contained in the soil. The source of nitrogen to plants was long a disputed question; it was at first supposed (by de Saussure) that it was obtained directly from the soil by the absorption of humus; but this was disproved, and Liebig then contended that it was derived directly from the atmosphere through the ammonia in rain water. This was then shown to be wholly inadequate; and Boussingault proved conclusively that plants do not take up nitrogen gas from the air. This was subsequently denied by Ville; but investigation at the Rothamstead agricultural station by Lawes and Gilbert definitely confirmed Boussingault’s results. At the same time they also proved very definitely that while grass and root crops deplete the soil of nitrogen, clover and other leguminous crops leave in the soil more nitrogen than was previously present, even when the entire, itself highly nitrogenous, leguminous crop is removed from the land. The improvement of lands for wheat production by rotation with clover had long ago become a practical maxim; but the cause was not understood until, in 1888, Hellriegel and Wilfarth announced that the variously-shaped excrescences or tubercles which had long been observed as frequently deforming the roots of legumes, are caused by the attacks of bacilli capable of absorbing the free nitrogen of the air and thus enabling the host-plant to acquire its needed supply by absorbing the richly nitrogenous matter thus accumulated in the excrescences. The minute rod-shaped organism was named _Bacillus radicicola_ by Beyerinck; _Rhizobium leguminosarum_, by A. Frank, who has published an extensive treatise on the subject.[57] [Illustration: FIG. 21.—Microscopic section of cell tissue, from a nodule of Square-pod pea, showing cells filled with Rhizobia.[58]] [57] Uber die Pilzsymbcose der Leguminosen, Berlin, 1890. [58] Original figure from drawing by O. Butler, Asst. in Agr. Dep’t Univ. of California.