The vegetative fibrils (mycelia) of several kinds of molds are constantly present in the soil, and while consuming the dead tissue of the higher plants, spread their own substance throughout the soil mass. The same is true of the subterranean or “root” mycelia of the larger fungi, toadstools, mushrooms, which are commonly found about dead stumps and other deposits of decaying vegetable and animal offal. All these being dependent upon the presence of air for their life functions, remain within such distance from the surface as will afford adequate aeration; the depth reached depending upon the perviousness of the soil and subsoil. In the humid region this will usually be within a foot of the surface, but in the arid may reach to several feet. Ultimately these organisms contribute their substance to the store of humus in the land. On the surface of moist soils we frequently find a copious growth of green fibrils, which may be either those of algæ, such as Oscillaria, or the early stages (prothallia) of moss vegetation. This vegetation has been credited with absorption of nitrogen from the air, thus enriching the soil; but later researches have shown this effect to be due to symbiotic bacteria (see above p. 156). _Animal Agencies._—Darwin first suggested that wherever the common earthworm (_Lumbricus_) finds the conditions of existence, it exerts a most important influence in the formation of the humous surface-soil layer; and the limitation imposed upon these conditions by the subsoil has doubtless a great deal to do with the sharp demarcation we often find between it and the surface soil. Briefly stated, the earthworm nourishes itself by swallowing, successively, portions of the surrounding earth, digesting a part of its organic matter and then ejecting the undigested earth in the form of “casts,” such as may be seen by thousands on the surface of the ground during or after a rain. Darwin (The Formation of Vegetable Mold, 1881), has calculated from actual observation that in humid climates and in a ground fairly stocked with these worms, the soil thus brought up may amount to from one-tenth to two-tenths of an inch annually over the entire surface; so that in half a century the entire surface foot might have been thus worked over. Aside from the mechanical effect thus achieved in loosening the soil, and the access of air and water permitted by their burrows, the chemical effects resulting from their digestive process, and the final return of their own substance to the soil mass; also their habit of drawing after themselves into their burrows leafstalks, blades of grass and other vegetable remains, renders their work of no mean importance both from the physical and chemical point of view. The uniformity, lack of structure and loose texture of the surface soil, especially of forests, as compared with subsoil layers of corresponding thickness, is doubtless largely due to the earthworms’ work. It has