It seems sufficient to describe here one of these pedigree-cultures, as the results of all four were similar. In the fall of 1886 I took nine large rosettes from the field, planted them together on an isolated spot in the garden, and harvested their seeds the next year. These nine original plants are therefore to be considered as constituting the first generation of my race. The second generation was sown in 1888 and flowered in 1889. It at once yielded the expected result. 15,000 seedlings were tested and examined, and among them 10 showed diverging characters. They were properly protected, and proved to belong to two new types. 5 of them were _lata_ and 5 _nanella_. They flowered next year and displayed all the characters as described in our preceding lecture. Intermediates between them and the general type were not found, and no indication of their appearance was noted in their parents. [550] They came into existence at once, fully equipped, without preparation or intermediate steps. No series of generations, no selection, no struggle for existence was needed. It was a sudden leap into another type, a sport in the best acceptation of the word. It fulfilled my hopes, and at once gave proof of the possibility of the direct observation of the origin of species, and of the experimental control thereof. The third generation was in the main a repetition of the second. I tried some 10,000 seedlings and found three _lata_ and three _nanella_, or nearly the same proportion as in the first instance. But besides these a _rubrinervis_ made its appearance and flowered the following year. This fact at once revealed the possibility that the instability of _lamarckiana_ might not be restricted to the three new types now under observation. Hence the question arose how it would be possible to obtain other types or to find them if they were present. It was necessary to have better methods of cultivation and examination of the young plants. Accordingly I devoted the three succeeding years to working on this problem.