Fat, determination of, 69, 75. Fecal and non-fecal members, colon group, 106. Fermentation tubes, 93. Ferrous sulfide in sludge and mud, 76. Ferrous iron, determination of, 47. Ferric iron, determination of, 48. Fineness, coefficient of, 8. G. Gelatin media, 94, 96. H. Hardness, determination of, 30. bicarbonate, 37. carbonate, 38. hydroxide, 38. non-carbonate, 34. temporary, 34. Hydrogen sulfide, determination of, 63. Hydrogen-ion determination, 94. I. Ignition, loss on, 30. Incubation, 99. Indol test, broth for, 107. Indicators, 36, 94, 107. Iodine and bromine, determination of, 61. Iron and Aluminium, separation, 57. analysis of, 79. Iron, determination of, 43. standards, 45. sulfate, determination of, 41. analysis of, 81. L. Lacmoid indicator, 36. Lead, determination of, 51, 55. Lime, analysis of, 80. Lithium, determination of, 60. Litmus reagent, 94. lactose-agar, 97. solution, 96. M. Manganese, determination of, 48. Materials, bacteriological, 93. Meat extract, 93. Media, culture (see “Culture media”), 94–7, 107. Methyl orange indicator, 37. Methyl red media, 107. test, 107. Microscopical bibliography, 91. examination, 89. Mineral analysis, 56. Moisture in sludge and mud, 74. Mud deposits, analysis, 73. N. Nessler’s reagent, color standards, 10. ammonia determination, 19. Nitrogen, 15. ammonia, 15. albuminoid, 20. Nitrogen, in sludge and mud, 74. nitrate, 23. nitrite, 22. organic, 21. total, 25. Nutrient media (see “Culture media”), 94, 107. O. Odor, 12. Organic nitrogen, 21. Oxygen consumed, 25. demand, biochemical, 71. dissolved, 65. in fresh and sea water (table), 68. P. Peptone, authorized brands, 93. Persulfate method (Mn), 48. Petri dishes, 93. Phenoldisulfonic acid method (nitrate), 23. Phenolphthalein indicator, 36, 94. Physical examination, 4. Pipettes, bacteriological, 93. Plating, bacteriological, 99. Platinum-cobalt color standard, 9. wire turbidity, 5. Potassium, determination of, 59. Presumptive tests, colon group, 102. R. Reactions of colon group, 108. Reaction of culture media, 94. of sludge and mud, 73. Reduction method (nitrate), 24. Relative stability method, 71. Residue on evaporation, 29. Results, expression of, bacteriological, 103. chemical examination, 14. color, 8. odor, 12. Results, interpretation of (bacteriological), 106. Routine procedure (bacteriological), 108. S.