Standard methods for the examination of water and sewageAmerican Public Health Association. Laboratory Section
Science
Standard methods for the examination of water and sewage
American Public Health Association. Laboratory Section
Sewage -- Analysis; Water -- Analysis
Results shall be expressed in the number of Standard Units of each kind
of micro-organism per cc. and also the total number of standard units of
all kinds per cc. The general directions as to significant figures given
under Turbidity shall apply also to the microscopical examination.
_Caution._—Many micro-organisms, especially some of those causing odors,
are so fragile that they are broken up in filtration, especially if the
agitation of the filtrate is too vigorous. A direct examination of a
fresh sample is therefore a useful supplementary procedure. For the same
reason the concentrate should not stand long before examination. Also
some organisms are carried by specific gravity to the top of the cell
which should be scrutinized as well as the bottom layer each time.
It is always better to examine the micro-organisms in the field when
possible, and for this purpose the sling filter has been devised
consisting of a metal funnel slung to a pivoted handle, with a disk of
wire gauze in the detachable lower end to support the sand. Filtration
is hastened by imparting a whirling motion to the whole and utilizing
the centrifugal force thus generated.
[Illustration: THE OCULAR MICROMETER.]
MICROSCOPICAL BIBLIOGRAPHY.
_a._ KEAN, A. L. A new method for the microscopical examination of
water: _Science_, Vol. 13, p. 132, 1889; _Eng. News_, pp. 21,
276, 1889.
_b._ SEDGWICK, W. T. Recent progress in biological water analysis: _J.
N. E. Water Works Assoc._, Vol. 4, pp. 50–64, 1889.
_c._ ——. A report of the biological work of the Lawrence Experiment
Station: _Examinations by the State Board of Health of water
supplies of Mass., 1887–90_, pt. 2, Purification of sewage and
water, pp. 793–862, 1890.
_d._ PARKER, G. H. Report upon the organisms, excepting the bacteria
found in the waters of the State: _Examinations by the State
Board of Health of water supplies of Mass., 1887–90_, pt. 1,
Examination of water supplies, pp. 579–620, 1890.
_e._ RAFTER, G. W. The microscopical examination of potable water, D.
Van Nostrand Co., New York, 1910. (Contains bibliography.)
_f._ CALKINS, G. N. The microscopical examination of water: _Report
Mass. State Board of Health_, pp. 397–421, 1892.
_g._ JACKSON, D. D. On an improvement in the Sedgwick-Rafter method for
the microscopical examination of drinking water: _Tech. Quart._,
Vol. 9, pp. 271–4, 1896.
_h._ WHIPPLE, G. C. Experience with the Sedgwick-Rafter method at the
Biological Laboratory of the Boston Water Works: _Tech. Quart._,
Vol. 9, pp. 275–9, 1896.
_i._ ——. Microscopy of drinking water, 3d ed., John Wiley & Sons, New
York, 1914. (Contains bibliography.)
BACTERIOLOGICAL EXAMINATION.
I. APPARATUS.
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