Studies on Fermentation: The diseases of beer, their causes, and the means of preventing themPasteur, Louis
Science
Studies on Fermentation: The diseases of beer, their causes, and the means of preventing them
Pasteur, Louis
Beer; Fermentation
This, however, is not the most striking inference from the facts which
the cultivation of these organisms revealed. The _mucor_ is evidently a
plant, at the same time _aërobian_ and _anaërobian_. If we had sown the
spore-bearing filaments of _mucor_ on slices of pear, lemon, or similar
fruit, we should have seen the spores germinate, tubes of mycelium
ramifying on the surface of the substratum, and reproducing sporiferous
aerial _hyphae_. In this case the plant would have effected all its
phenomena of nutrition by absorbing oxygen and emitting carbonic acid,
after the manner of animals, as, in our essay on the organic corpuscles
which exist in a state of suspension in the atmosphere, we have shown to
be the case generally with fungoid growths. Under these circumstances,
the only sugar decomposed would have been a quantity equivalent to that
assimilated in forming the cellulose of the young tissues of the fungus,
or in entering into combination, either with the elements of ammonia or
with the sulphur of the sulphates, or the phosphorus of the phosphates,
to form the albuminous substances of the interior of the cells.[77] In
this case the sugar used up would furnish no alcohol, or at least, if
alcohol were formed, it would be decomposed immediately. All _aerial_
growths take place in the same manner; and such is the nature of
nutrition and life in all the larger forms.
In our flasks, on the other hand, the life of the little plant functions
quite differently. Deprived of oxygen, or having at its disposal but an
insufficient quantity of that gas, after a life of activity in contact
with air, it can, nevertheless, live apart from the direct action of
that element, and the combinations to which it gives rise. On the other
hand, we see all the signs of alcoholic fermentation appear; that is, a
notable proportion of sugar, in comparison with the weight of solid
matter assimilated and fixed by the plant, is decomposed into alcohol
and carbonic acid gas; and this decomposition continues as long as life
itself continues in the cells, and they remain submerged, this last
condition being effected by the decantation of the liquid and its
deposit into the test-glass. Along with the disappearance of the
phenomena of vital activity in the cells, the fermentation ceases
absolutely, or at least is no longer visible externally, by reason of
its extreme feebleness. The cells then assume an old, shrivelled,
worn-out appearance, with irregular outlines and granular markings.
Their life is merely suspended, however, not extinct; for if they be
supplied once more with oxygen, and suffered to exist under the
influence of that gas, they will vegetate again, and become capable of
producing fermentation afresh, even after having been excluded from the
air for a considerable time.
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