Studies on Fermentation: The diseases of beer, their causes, and the means of preventing themPasteur, Louis
Science
Studies on Fermentation: The diseases of beer, their causes, and the means of preventing them
Pasteur, Louis
Beer; Fermentation
In concluding our study of the vegetation of _mucor_ as a mould and
_mucor_ as a ferment, we may again remark that the most striking
analogies also exist between the preceding observations and those we
have seen in the case of _penicillium_, _aspergillus_, and _mycoderma
vini_. These latter plants do not furnish alcohol or carbonic acid gas
by direct fermentation of the sugar, as long as we let them vegetate
with plenty of air at their disposal. Once submerged, however, their
vital aspect changes; on the one hand the cells or filaments of the
mycelia evince a tendency to become larger; on the other hand there is a
tendency to greater closeness in these latter, and, consequently, a
transition to the state of _conidia_. Lastly, there is a correlative
budding of cells, accompanied by a formation of alcohol and liberation
of carbonic acid gas; in short, all the ordinary signs of alcoholic
fermentation.
The principal difference in the case of _mucor_ consists in this, that
the vegetation of this latter, under the conditions of insufficient
aeration or none at all, is more decided, both as to extent and
duration.
It may be thought that all the varieties of _mucor_ are capable of
yielding the kind of ferment that we have just mentioned. But this is
not the case; and here we have another striking proof of the great
physiological differences presented by forms of vegetation so intimately
connected with each other that, in botanical classifications, they must
be put as closely as possible together. Of this fact we have the most
striking example in _mycoderma vini_ and the alcoholic ferments,
properly so called, which so closely resemble each other in form and
development that they might be supposed to be identical, at least,
according to our present knowledge, but which differ so widely in their
physiological aspects.
On November 17th, 1873, we found a very beautiful specimen of _mucor
mucedo_ on a pear, under a glass bell jar. It was a mass of perfectly
straight filaments, simple and isolated, very large in comparison with
those ordinarily met with, each terminating in a sporange, identical to
that of _mucor mucedo_, and proportionately well developed. We are able
to distinguish _mucor racemosus_ from _mucor mucedo_ only by the
circumstance of its having on its sporange-bearing hyphæ lateral
branches which also terminate in sporanges.
We sowed only one of the terminal heads of the large erect hyphae in
some wort, in which it soon produced an abundant mycelium, but without
the least appearance of gas. For a very long time, up to January 7th,
1875, we studied the developments of this organism, which remained all
the time perfectly pure, in consequence of our having cultivated it in
one of our two-necked flasks on pure wort.
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