Studies on Fermentation: The diseases of beer, their causes, and the means of preventing themPasteur, Louis
Science
Studies on Fermentation: The diseases of beer, their causes, and the means of preventing them
Pasteur, Louis
Beer; Fermentation
Plate X. represents this ferment, at the commencement of fermentation in
cherry juice. In the course of a short time there is nothing to be seen
but cells of comparatively small size, disjointed and round or oval, and
filaments comparatively short and slender. This appearance is indicated
in our drawing by the cells _a_, _a_, _a_. As these latter forms
multiply with great rapidity, we soon have to search widely over the
microscopic field before we find any of the long forms from which they
spring. Instead of the forms given in Plate X., we have only those
represented in Plate XI. In other words, the aspect of these ferments
changes daily, from the very commencement of fermentation. Thus the
yeast would appear to grow smaller, coincidently with the progress of
fermentation passing from a condition in which it consists of large
cells and long ramified filaments, to a condition in which the cells are
small and the filaments short. These changes are principally due to an
alteration in the method of budding and in the life-processes of the
yeast, which speedily exhibits itself when the air supply is reduced,
and not through any intermixture of foreign ferments. So, at least, all
our observations up to the present time lead us to believe. As soon as
the oxygen has been absorbed the cells which form are oval or globular,
and the filaments do not lengthen or become so plump.
[Illustration: Plate 11. accharomyces Pastorianus, in course of Regular
Growth.]
This is, however, not the only cause of these changes in form and
aspect, although the presence of air, in greater or less quantity, has a
marked influence on the earlier developments of yeast; there is another
circumstance to be taken into account, difficult indeed to state
shortly, but which is demonstrated clearly by the microscope, and is
connected with the actual state of the germ cells. As a general rule the
budding of a cell is not an identical process when the cell is quite
young, and when it has become exhausted from want of nourishment.
Between these two conditions there is a difference which may be compared
with that which exists, for example, between a newly-formed grain which
would not germinate, and the same grain matured by rest, if we may use
the expression, that is, which has been kept long enough for its
germination to be possible. In other words, and as far as our subject is
concerned, we are not to expect that, by reviving our old yeast cells
and putting them to grow with abundance of air, in a saccharine,
nutritive medium, we shall obtain the appearance of the earlier
developments of the germ-cells on the surface of sweet and acid fruits.
We see this clearly in Plate VII., the right-hand half of which
represents the recruited budding of cells, such as those represented in
the left-hand half, in a medium peculiarly adapted to their vitality,
_and in the presence of much air_. As regards the length and size of
filaments and cells, there is little appreciable difference between the
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