We may observe here that these conditions seem to be peculiarly adapted to show the character of the interior sporulation of the cells discovered by Dr. Rees. Notwithstanding this we have never succeeded in finding it distinctly, under these circumstances. The fact which should claim all our attention, we repeat, is, that this exhausted, shrivelled-up, aged-looking yeast preserves its faculty of germination for several years; that, moreover, this faculty may be aroused by placing it in aerated nutritive media, in which case it will exhibit all the peculiarities which, under similar conditions, characterize some of the germ-cells found on the surface of our sweet domestic fruits. In other words, this yeast, instead of multiplying, as it always does in the course of several growths in saccharine musts, in the form of cells which detach themselves readily as soon as they have nearly attained the form and size of the mother-cells, begins to shoot out into such beautiful forms as those of _dematium pullulans_, producing like that ferment long, well-grown, branching filaments, as well as plump and frequently pyriform cells, as represented in Plate X. The following figures (33 to 37) and descriptions of the observations to which they relate will furnish fresh proofs of our assertions. In these figures we see _saccharomyces pastorianus_, which has been exhausted in sweetened water or in yeast-water, undergo revival in saccharine musts, give rise to elongated, branching, pear-shaped forms, such as belong to the original ferments of fruits, and afterwards assume the most minute forms that we find in fermentations progressing or completed. Let us examine Fig. 33. The history of this growth is as follows:— Some spontaneous yeast which, after repeated cultivation, had acquired the aspect represented in Plate XI.—which aspect the _saccharomyces pastorianus_ generally assumes under these circumstances—was exhausted in sweetened water, and subsequently revived in must at 10° C. to 11° C. (51° F.). At this temperature germination was not very marked before the end of eight days; at a temperature of 20° C. (68° F.), it only took three days, under similar conditions. The sketch includes but one of the long branches from which the ferment cells and the budding joints took rise, but there were a great number more. Some of the forms represented in the figure bear a striking resemblance, it appears to us, to some of those of _dematium_, in Plate IX.; and even we may trace out the several peculiarities of form which distinguish the figures in the latter plate. [Illustration: Fig. 33.]