Studies on Fermentation: The diseases of beer, their causes, and the means of preventing themPasteur, Louis
Science
Studies on Fermentation: The diseases of beer, their causes, and the means of preventing them
Pasteur, Louis
Beer; Fermentation
fermentations of wort either a distinctly “high” ferment, or a
distinctly “low” one, properly so called; nor, further, have we ever
obtained either one of these distinct kinds, with its industrial
characteristics, in experiments on the ferments of fruits. What, then,
was the origin of the “high” and “low” ferments now used by brewers?
What was the nature of their original germs? These are questions which
we are unable to answer, but we are very much inclined to think that we
have here another example of the modifications which plants as well as
races of animals undergo, and which become hereditary in the course of
prolonged domestication. We know nothing of corn in its wild state, we
cannot tell what its first grain was like. We know nothing of the
silk-worm in its original state, and we are ignorant of the characters
of the race that furnished the first egg.
These reflections may seem to favour the supposition that there is a
real difference between “high” yeast and “low” yeast, and that both of
these differ from spontaneous ferments and the ferments of domestic
fruits. These are propositions demanding most careful consideration, for
it is generally admitted that these ferments become intermixed, that
their morphological differences are merely a question of medium, and
that the transition of one to the other is a simple matter. The
following facts seem to contradict such statements.
[Illustration: Fig. 39.]
“High” Ferment.—Fig. 39 represents some “high” yeast taken from a
deposit after fermentation, and Fig. 40 the same yeast in course of
propagation in some aerated wort. In comparing “high” yeast with other
alcoholic ferments at the same stage of development, there are three
points which are especially striking: the diameter of its cells is
relatively large, their general aspect is rounder, and when they are
undergoing propagation their mode of budding produces a markedly
ramified appearance, so that the cells always occur in clusters and
branches. Fig. 40 gives a very exact idea of these characters. To
investigate satisfactorily the branching habit of growth peculiar to
this ferment we should examine it during the first few hours of its
propagation, when, under the influence of the oxygen dissolved in the
fermentable liquid, its vital activity is greatest. Later on, often on
the day following the sowing, the groups become disconnected, and at the
end of the fermentation the cells have quite separated from each other,
not more than 2 or 3 per cent. remaining united, and even these in
groups of not more than two cells together. This is represented in Fig.
39.
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