Studies on Fermentation: The diseases of beer, their causes, and the means of preventing themPasteur, Louis
Science
Studies on Fermentation: The diseases of beer, their causes, and the means of preventing them
Pasteur, Louis
Beer; Fermentation
Multiplying afresh in the fermented medium, under the influence of the
air, they form a kind of mycodermic film on the liquid surface, or a
ring round the sides of the flask, on a level with the liquid. This
development might often be mistaken for _mycoderma vini_ or _cerevisiæ_;
in reality there is not a single cell of _mycoderma_ formed. If we sow a
trace of the new growth in a saccharine medium it will behave exactly as
yeast would, budding and multiplying, and setting up fermentation in the
liquid. And thus, in spite of its mycodermic aspect, this growth is
nothing but yeast, since it gives rise to true alcoholic fermentation;
but it is a kind of yeast which, under the foregoing conditions, lives
after the manner of fungoid growths, absorbing the oxygen of the air and
emitting carbonic acid gas. It appears on the surface of all fermented
liquids, especially those which, like beer, contain carbohydrates, and
its quantity is the greater, and its action the more rapid, in
proportion as it has more perfect access to the air. We have termed this
yeast _aërobian ferment_ or _fungoid ferment_.
It may easily be understood how this kind of production has escaped
notice up to the present time. The conditions of our experiment were, in
many respects, novel; a saccharine liquid had never before been caused
to ferment by means of pure yeast, absolutely free from foreign germs; a
fermented liquid had not previously been exposed to contact with pure
air for an indefinite time. On the other hand, all ordinary fermented
liquids, when left to themselves in contact with air, are a ready prey
to _mycoderma vini_ or _aceti_ at their surface, and then give rise to
true fungoid growths. The appearance of these organisms, which always
takes place soon, has thus constantly concealed or prevented the
development of the true aërobian ferments. In repeating the experiment
described any alcoholic ferment may be used, and each one will be found
to produce its own peculiar fungoid form of ferment. Another point
worthy of notice is that these aërobian ferments, when they put forth
buds in the act of fermentation, reproduce the forms of the original
ferment, at least apparently so. In this respect they cannot be
distinguished, notwithstanding the fact, surprising as it seems, that
the two kinds of ferments are not identical. If we operate on a “low”
yeast its aërobian ferment will differ physiologically from the ferment
from which it sprung, presenting various special peculiarities which are
not to be found in the original “low” yeast. In most of our experiments
we have found the new aërobian ferment to be similar in its action to
“high” yeast, rising to the surface, and producing a beer which
possesses a greater fragrance than beer brewed with the identical “low”
yeast from which it was derived. Lastly, the properties of an aërobious
ferment are not peculiar to first growth, but are hereditary; by
repeating the growth of the first aërobian ferment we do not cause them
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