Studies on Fermentation: The diseases of beer, their causes, and the means of preventing themPasteur, Louis
Science
Studies on Fermentation: The diseases of beer, their causes, and the means of preventing them
Pasteur, Louis
Beer; Fermentation
On November 25th we made another examination and sketch of the ferment,
the appearance of which did not differ materially from that given above.
The general appearance was the same, consisting mostly of globules
joined together in clusters of two or three or more. No separation, such
as occurs in the case of ferment formed in natural worts, had taken
place. The ferment, moreover, was very irregular, and comprised cells of
all sizes. We sowed some of it in a flask of pure wort. On November 26th
there was no apparent development: on November 27th, however, not more
than forty-eight hours after impregnation, there was a considerable
deposit of white ferment at the bottom of the liquid, and fermentation
was so active that the surface of the liquid was covered with an
abundant froth. This shows us the wonderful vitality and recuperative
power possessed by germs which, left to themselves for about four
months, revived so readily. It proves too that the reviving influences
took effect on some aërobian ferment. From the mode of life of this
latter being similar to that of a surface fungoid growth, it does not
become exhausted as the cells of ordinary ferment do. Now the cells
which, sown on August 6th, had become exhausted by prolonged stay in the
mineral liquid, and were almost inert, would have required several days
for their revival; but in the experiment described the revival was
rapid, and this rapidity proves, as we have said, that the revival must
have taken place in cells of aërobian ferment.
[Illustration: Fig. 50.]
Taking some fresh yeast from the bottom of the liquid we examined and
made a sketch of it (Fig. 50). The field was filled with round and oval
cells, jointed and ramified filaments, budding and multiplying in the
most remarkable manner, reminding us of the germination of the cells of
yeast exhausted in sweetened water, and also of the germination in the
form of _dematium pullulans_ of certain germ-cells which are spread over
the surface of sweet, domestic fruits. We could never grow tired, as we
wrote it in our original notes, of sketching this beautiful plant, which
establishes very clearly a transition between one of the best defined
cellular ferments, viz., _saccharomyces pastorianus_, and certain forms
of very common fungoid growths, those of _dematium_, and even of the
most common mould, _mucor mucedo_ or _racemosus_, when it vegetates
beneath the surface of a liquid and acts as a ferment.[108] We have
here, as in these cases, filamentous chains branching into other similar
chains, composed of more or less elongated cells, which at length fall
off and germinate exactly as the conidia-bearing _hyphae_ of _mucor_ do.
The aërobian ferment of “high” yeast, in whatever medium we cultivated
it, presented no peculiarity, as far as its forms were concerned. It was
composed of cells of spherical shape, like ordinary “high” yeast, and
germinated in the same way as the latter.
[Illustration: Fig. 51.]
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