Studies on Fermentation: The diseases of beer, their causes, and the means of preventing themPasteur, Louis
Science
Studies on Fermentation: The diseases of beer, their causes, and the means of preventing them
Pasteur, Louis
Beer; Fermentation
rapidity, and darting backwards and forwards; these were probably
identical with the longer ones, and possessed greater freedom of
movement, no doubt in consequence of their greater shortness. Not one of
these vibrios could be found throughout the mass of the liquid.
We may remark that as there was a somewhat putrid odour from the deposit
in which the vibrios swarmed, the action must have been one of
reduction, and no doubt to this fact was due the greyish coloration of
the deposit. We suppose that the substances employed, however pure,
always contain some trace of iron, which becomes converted into the
sulphide, the black colour of which would modify the originally white
deposit of insoluble tartrate and phosphate.
But what is the nature of these vibrios? We have already said that we
believe that they are nothing but the ordinary vibrios of putrefaction,
reduced to a state of extreme tenuity by the special conditions of
nutrition involved in the fermentable medium used; in a word, we think
that the fermentation in question might be called putrefaction of
tartrate of lime. It would be easy enough to determine this point by
growing the vibrios of such a fermentation in media adapted to the
production of the ordinary forms of vibrio; but this is an experiment
which we have not ourselves tried.
One word more on the subject of these curious beings. In a great many of
them there appears to be something like a clear spot, a kind of bead, at
one of their extremities. This is an illusion arising from the fact that
the extremity of these vibrios is curved, hanging downwards, thus
causing a greater refraction at that particular point, and leading us to
think that the diameter is greater at that extremity. We may easily
undeceive ourselves if we watch the movements of the vibrio, when we
will readily recognize the bend, especially as it is brought into the
vertical plane passing over the rest of the filament. In this way we
will see the bright spot, the head disappear, and then reappear.
The chief inference that it concerns us to draw from the preceding facts
is one which cannot admit of doubt, and which we need not insist on any
further—namely, that vibrios, as met with in the fermentation of neutral
tartrate of lime, are able to live and multiply when entirely deprived
of air.
§ V.—Another Example of Life Without Air—Fermentation of Lactate of
Lime.
As another example of life without air, accompanied by fermentation
properly so called, we may lastly cite the fermentation of lactate of
lime in a mineral medium.
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