Studies on Fermentation: The diseases of beer, their causes, and the means of preventing themPasteur, Louis
Science
Studies on Fermentation: The diseases of beer, their causes, and the means of preventing them
Pasteur, Louis
Beer; Fermentation
It is very remarkable that some of the _torulæ_ in Fig. 12 are not
ferments; they do not cause sugar to decompose into alcohol and carbonic
acid, any more than the _mycoderma vini_ does; but, nevertheless, there
may be an absolute similarity in aspect, development, shape, and size
between the alcoholic ferment, properly so called, and these
_torulaceæ_.
We must here cite a case in proof. On May 28th, 1872, in one of the
rooms of our laboratory, we broke the fine points of a series of flasks,
similar to those used in our previous experiments, containing must of
grapes and deprived of air. We then closed up the ends immediately after
the sudden entrance of the exterior air. One of these flasks developed
only one kind of organism, which belonged to the _torulaceæ_. On June
7th this was sufficiently abundant to cover all the sides with a white
deposit, and the surface of the liquid appeared quite free of any
_mycoderma vini_. To assure ourselves that we were actually dealing with
one kind of _torulæ_, unattended by fungoid growths, we waited until
June 14th, but the aspect of things remained unchanged. On that day we
opened the flask; there was no escape of gas to indicate that the
interior pressure was greater than the exterior. We then subjected the
plant to microscopic examination. It was quite homogeneous, and formed
of a mass of cells, absolutely identical in aspect and size with old
cells of ordinary yeast (Fig. 13).
[Illustration: Fig. 13.]
We distilled all the liquid, of which there was about 100 c.c., (3-½ fl.
oz.) without obtaining any trace of alcohol in our distillate; we
collected 33 c.c. (about an ounce) of the distillate, which we distilled
again, and we submitted the distillate a third time to distillation; but
even then there were no more signs of the presence of alcohol than there
had been in our first distillate.[46]
We may safely conclude that our _torula_, in the course of its
development in must, with a weight that would have been very
appreciable, did not produce, by its action of multiplication 1/10,000
of 1 c.c. of alcohol.
Under the following conditions we obtained a slightly different result,
which, nevertheless, confirmed the preceding one.
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