Studies on Fermentation: The diseases of beer, their causes, and the means of preventing themPasteur, Louis
Science
Studies on Fermentation: The diseases of beer, their causes, and the means of preventing them
Pasteur, Louis
Beer; Fermentation
If we study the vegetation of _aspergillus glaucus_ with this
preconceived idea, we shall soon recognize the fact that these spherical
forms of mycelium are the result of a greater or less deprivation of
air. The filaments of this mycelium which develop freely in the aerated
liquid are young and transparent, small in diameter, and exhibit the
ordinary ramifications. Those which are situated about the centre, in
the denser or more complicated parts, to which the oxygen cannot
penetrate in consequence of its absorption by the surrounding parts, are
more granular in appearance as well as larger, and inclined to develop
swellings. We can observe no _conidia_[63] on these filaments, but we
may say that they are on the point of appearing, for the spherical
segments often tend to assume an appearance of close jointing, as when
they take the form of those rows of swelling, or cells, which has given
rise to the idea of the _chaplets of the conidia-cellules_. This is
represented in the accompanying sketches (Fig. 21), which we have
purposely contrasted with two similar ones which relate to the _mucor_,
of which we shall soon speak. The _conidia_ of these latter are very
remarkable, and their fermentative character becomes apparent as soon as
their growths are deprived of air.
[Illustration: Fig. 21 A. Growth with abundant air-supply at the edge of
the mycelium crust.]
[Illustration: Fig. 21 B. Growth with reduced air-supply in the central
and deeper parts of the mycelium.]
[Illustration: Fig. 21 C. Growth with abundant air-supply at the edge of
the mycelium tuft.]
[Illustration: Fig. 21 D. Growth with reduced air-supply in the central
and deeper parts.]
It is scarcely necessary to add that in these vegetations of
aspergillus, which were accompanied by a corresponding alcoholic
fermentation, it was impossible to find cells of yeast; and that,
notwithstanding this, the liquid was so adapted to ordinary alcoholic
fermentation, that, when we added a small quantity of yeast to it, in
the course of a few hours, a most active alcoholic fermentation declared
itself.
We may give some other facts relating to a crop of _aspergillus glaucus_
which was also grown in ordinary hopped wort, and which was left to
itself for a year.
A two-necked flask, holding 300 c.c. (rather more than 10 fl. oz.) was
prepared and impregnated on December 21st, 1873, and was then placed in
an oven at a temperature of 25° C. (77° F.). The fungoid growth
developed in isolated tufts, which subsequently united, but without
entirely covering the surface of the wort. A few tufts also vegetated at
the bottom of the liquid; those on the surface soon became surrounded by
large bubbles of gas.
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