There might perhaps be room for some fear that the conditions of growth in our flasks were not favourable to the simultaneous appearance of these common fungoid growths along with the _mycoderma vini_. On June 24th, 1872, we sowed, in three flasks of sugared yeast-water, prepared as before—in the first, _mycoderma vini_, together with _penicillium glaucum_; in the second, _mycoderma vini_, together with _mucor mucedo_; in the third, _mycoderma vini_ alone. We effected this by plunging the platinum wire, which we used for impregnating the liquids, into the pure film of _mycoderma vini_, and then touching with the wire the sporanges of the other fungus. On June 29th, we saw on the surface of our first flask some green patches of _penicillium_, along with some spots of _mycoderma vini_; in the second flask a voluminous mycelium of _mucor mucedo_, distended by large bubbles, had risen to the surface of the liquid, and was entirely covered by a film of _mycoderma vini_. As for the liquid in the third flask, there were only a few spots of very pure _mycoderma vini_. This last flask, after being kept in an oven at 25° C. (77° F.) for several months, still contained nothing but _mycoderma vini_, unmixed with any other fungoid growth whatever. We may therefore be sure that _mycoderma vini_, vegetating on the surface of liquids adapted to its nutrition, in contact with air deprived of its germinating dust, will not present the least sign of a transformation into any of these other common fungi, or into yeast, however long may be the duration of its exposure to contact with that pure air. We may now return to that feeble and limited production of carbonic acid gas and alcohol, the formation of which we have shown experimentally to take place at a high temperature, after submerging the film of _mycoderma vini_.[67] There can be no doubt that we have here a phenomenon similar in every point to that presented by _penicillium_ and _aspergillus_, which we studied in the preceding paragraph. When the germs or jointed filaments of _mycoderma vini_, growing on a saccharine substratum in contact with the air, are in the full activity of life, this activity is carried on at the expense of the sugar and other materials in the liquid, in the same way that animals consume the oxygen of the air and evolve carbonic acid. The consumption of the different materials is attended with a proportionate formation of new materials, development of structure, and reproduction of organisms.