Studies on Fermentation: The diseases of beer, their causes, and the means of preventing themPasteur, Louis
Science
Studies on Fermentation: The diseases of beer, their causes, and the means of preventing them
Pasteur, Louis
Beer; Fermentation
Our experiments were conducted in the following manner:—In some flat
porcelain basins, we grew some pure _mycoderma vini_[70] on fermented
liquids, such as wine or beer, or on artificially vinous liquids, such
as alcoholized yeast-water, taking care to boil these liquids previously
to kill any germs of yeast or other organism that they might contain.
The basins themselves, as well as the plates of glass with which they
were to be covered, were plunged into boiling water just before they
were wanted for use. As soon as the film of mycoderma had become well
developed and thick, and even wrinkled—a process requiring not more than
two or three days during summer heat—we decanted the subjacent liquid,
by means of a siphon, so as to leave the film on the bottom of the
basin. We then diffused the whole mass of efflorescence in a saccharine
liquid that had been boiled and afterwards cooled down in a closed
vessel; generally, we used wort or must preserved by Appert’s process.
After that, we emptied the mixture of saccharine liquid and
efflorescence into long-necked flasks that had likewise been previously
heated, as also had the funnels used in the process of transference.
It seemed to us that experiments conducted with all these precautions
must be free from causes of error. It was true that we were working more
or less in contact with atmospheric air, but all that we had to fear for
the soundness of the conclusions which we might draw was the presence of
germs of alcoholic ferment, and we considered how few of these there are
amongst floating particles of dust. Consequently, if we succeeded in
observing the advent of yeast in each of the long-necked flasks,
accompanied by an active alcoholic fermentation, we thought that we
might, without danger of error, admit as a fact the transformation of
cells of mycoderma into cells of yeast. Again, we thought that we should
probably find in the forms of the cells of yeast which were directly
derived from the cells of mycoderma, a more or less elongated structure,
which would be a convincing proof of the transformation that we were
seeking, if, indeed, such transformation were possible.
Strange to say, everything happened in a manner that seemed to realize
our expectations. The saccharine worts in the flasks in which we had
mixed and submerged the mycoderma, fermented in the course of a few
days; the yeast first appeared in elongated shapes; lastly, we could see
under the microscope that many of the cells or jointed filaments of
mycoderma were inflated and presented the appearance of undoubted
gradations between their natural state and that of the cells of yeast
which soon formed part of the deposit in the vessels. In spite of all
this, however, we were the victims of an illusion.
Public-domain text, read in full here on John Shaqi.
Reviews
Reviews
No reviews yet
Be the first to share your thoughts on this work.
Elsewhere in the archive
Join the Discussion
Join the discussion
Sign in to leave a comment or review.
Sign InorCreate an account