at 110°, finding 6.15 per cent. in 100 parts of Oolong, 6.58 in Congou, and 6.30 in Green tea, while from one sample of India he extracted only 5.10 per cent., proportions six times greater than had been heretofore obtained by any previous analysis. Next testing every soluble substance for nitrogenous matter, he proceeded by successive eliminations to ascertain the quantity of theine in 27 other different samples and found that Green teas contained on an average 10 per cent. of water, and Black only 8 per cent., and also that the latter contained about 43.2 of matter soluble in boiling water while the former averaged as high as 47.1, and that this soluble matter yielded only 4.35 of nitrogen in Black teas, and 4.70 per cent. in Green. It remaining to be determined whether this large percentage of nitrogen was wholly due to the theine or in part to some other principle, he next found that the precipitate with sub-acetate of lead contained no apparent quantity, and then testing the theine by a modification of Mulder’s process obtained from Green tea an average of 2.48 per cent., and from a mixture of Green and Black 2.70. But greatly as these quantities exceed those of all other chemists, they were still unable to account for the whole amount of nitrogen found in the infusion, so by adding mere acetate of lead and ammonia, separating them by filtration, and passing through it a current of sulphuretted hydrogen to precipitate the lead, and evaporating the liquid with a gentle heat he obtained an abundant supply of crystals of theine. This supply he still further increased by re-evaporation until the whole amounted to 3.48 per cent. of the entire. There still remaining a syrup containing some theine it was precipitated with tannic acid, the result being added to that already crystallized it yielded a total of 5.84 from Green tea in the natural state and 6.21 in the dried leaf. These experiments being further continued by boiling the exhausted leaves with potash, it showed a presence of caseine to the extent of 28 per cent. of the mass, the proportion of the latter substance in the raw leaf being only 14 to 15.