Tea-extract yields a comparatively large quantity of ammonia when it is
boiled with potash and permanganate of potash, and it is probable that
this character may prove very valuable also in testing the purity of
tea, for which purpose a solution containing about 10 per cent. of solid
potash, free from ammonia and nitrogenous matter, is required and easily
obtained. Ten grams of this solution of potash is put into a small
flask-retort, working in an oil-bath and connected with a small
condenser; the whole apparatus to be carefully freed from the last
traces of ammonia, which is best accomplished by distilling the water
through it, after which from 5 to 10 parts of the tea infusion are
poured into the retort, which is then corked up and heated in an
oil-bath to 150 C. Having been maintained for a short time at this
temperature it is then lifted out of the bath and some pure water poured
into the retort, which is again to be heated in the oil-bath. More than
half of the water is then distilled over and in this manner the “free
ammonia” is obtained from the distillate. When this has been
accomplished some of the potash and permanganate solution is added to
the contents of the retort and distilled so as to yield a distillate
containing “albuminoid ammonia,” the result being as follows in pure
teas:—
Free ammonia, 0.28 milligrams.
Albuminoid ammonia, 0.43 “
—————
Total, 0.71 milligrams.
While the extract from a sample of spurious leaves yielded of
Free ammonia, 0.20 milligrams.
Albuminoid ammonia, 0.295 “
—————
Total, 0.495 milligrams.
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