It is to be noted that at an early stage the gall-bladder is derived from the main duct close to the intestine, the latter duct being very short. Later on the common duct grows in length, making the liver more and more a gross anatomical organ distinct from the intestine. The cystic duct develops as the result of a similar increase in length of the cystic diverticulum. The two principal secondary branches of the hepatic duct give origin to sprouts or buds. These are derivatives of the hypoblastic cells of the larger ducts and may from the beginning be hollow, possessing a lumen continuous with that of the parent duct (Selachians, Amphibians). In warm-blooded animals these sprouts are at first solid, forming the s. c. _hepatic cylinders_, and only subsequently become hollowed out with the further development of the biliary duct system of the liver. The rapid growth of the organ leads to a great increase in the number of the hepatic cylinders. They spread out on all sides, finally coalescing with adjacent buds so as to form an interlacing network whose meshes are filled by blood vessels. After the hepatic cylinders have become canalized they preserve the same arrangement, hence the resulting biliary capillaries of the adult form an anastomosing network. Amphioxus and the amphibians have a single hepatic outgrowth (Fig. 49). In the Selachians the liver arises as a ventral outgrowth at the hinder end of the foregut immediately in front of the vitelline duct, thus bringing the liver from the beginning into close proximity with the vitelline veins entering the heart. Almost as soon as formed the outgrowth develops two lateral diverticula, opening into a median canal. The two diverticula are the rudimentary lobes of the liver and the median canal uniting them is the rudiment of the common bile-duct and gall-bladder. In the Teleosts the liver arises quite late (in the trout about the 25th day) as a solid outgrowth from the intestinal canal close to the heart. In the Amniota the liver arises in the same position as in the Anamnia, but, at least in birds and mammals, shows its bifurcation almost, if not quite, from the start. The two forks embrace between them the omphalo-mesenteric or vitelline veins just before they empty into the sinus venosus of the heart. In the chick the liver appears between the 56th and 60th hour, the right fork being always of greater length but less diameter than the left. The hepatic outbud in the rabbit appears during the 10th day, and during the 11th day begins to send out branches. In man, as above stated, the bud appears well marked in embryos of 3 mm. [Certain adult variations make it appear possible that there are two human embryonic hepatic buds, a cranial and a caudal, as is the case in birds.]