There are thus three principal phases in the development of _T. cruzi_ in _Triatoma megista_: (1) A multiplicative phase (_Leishmania_-like) in the stomach of the bug, (2) a crithidial phase, which is also multiplicative, in the hind-gut, and (3) a trypanosome phase, which is “propagative,” and apparently passes through the wall of the alimentary canal into the body cavity and so into the salivary glands. [Illustration: FIG. 36.--_Trypanosoma cruzi_. Forms found in the salivary glands of _Triatoma megista_. (After Chagas.)] Brumpt found that _T. cruzi_ could live in _Cimex lectularius_, _C. boueti_, and _Ornithodorus moubata_. The _Cimex_ fæces may be infective. Blacklock found multiplication of the parasite in _C. lectularius_. _Culture._--The trypanosome can be cultivated on Novy-MacNeal’s blood agar, and the cultural forms resemble those described in the bug. _Possible Reservoir._--Chagas thinks that probably the armadillo or “tatu” (_Dasypus novemcinctus_) may be the reservoir of _T. cruzi_. He also thinks that _Triatoma geniculata_ is a transmitter; it lives in the burrows of the armadillo. Other carriers may be _Triatoma infestans_ and _T. sordida_. _Clinical Features._--The trypanosomiasis of Brazil, produced by _T. cruzi_ and spread by _Triatoma_ spp. has received various names, such as oppilação, canguary, parasitic thyroiditis, and coreotrypanosis. It is also known as the human trypanosomiasis of Brazil, South American trypanosomiasis, and Chagas’ disease. Chagas[88] reports two principal forms--acute and chronic. The _acute infection_ is rare, and is characterized by increase in the volume of the thyroid gland, pyrexia, a sensation of crackling in the skin, enlarged lymphatic glands in the neck, axilla, etc., while the liver and spleen are increased in volume. Sclerosis of the thyroid gland is found at autopsy and fatty degeneration of the liver. During an attack of fever, trypanosomes are found in the blood. The acute form was only observed in children. [88] _Brazil Medico_, Nov. 15, 1910. Longer account in _Mem. Inst. Oswaldo Cruz_, iii, pp. 219–275. See _Sleep. Sick. Bull._, Nos. 35 and 40.