Thus from the pith of the new bud of the horse-chesnut five vessels pass out through the circle of the placental vessels above described, and carry with them a minuter circle of those vessels; these five bundles of vessels unite after their exit, and form the footstalk or petiole of the new five-fingered leaf, to be spoken of hereafter. This structure is well seen by cutting off a leaf of the horse-chesnut (Aesculus Hippocastanum) in September before it falls, as the buds of this tree are so large that the flower may be seen in them with the naked eye. After a time, perhaps about midsummer, another bundle of vessels passes from the pith through the alburnum or sap-vessels in the bosom of each leaf, and unites by the new bark with the leaf, which becomes either a flower-bud or a leaf-bud to be expanded in the ensuing spring, for which purpose an apparatus of placental vessels are produced with proper nutriment during the progress of the summer and autumn, and thus the vegetable becomes annually increased, ten thousand buds often existing on one tree, according to the estimate of Linneus. Phil. Bot. The vascular connection of vegetable buds with the leaves in whose bosoms they are formed is confirmed by the following experiment, (Oct. 20, 1781.) On the extremity of a young bud of the Mimosa (sensitive plant) a small drop of acid of vitriol was put by means of a pen, and, after a few seconds, the leaf in whose axilla it dwelt closed and opened no more, though the drop of vitriolic acid was so small as apparently only to injure the summit of the bud. Does not this seem to shew that the leaf and its bud have connecting vessels though they arise at different times and from different parts of the medulla or pith? And, as it exists previously to it, that the leaf is the parent of the bud? This placentation of vegetable buds is clearly evinced from the sweetness of the rising sap, and from its ceasing to rise as soon as the leaves are expanded, and thus compleats the analogy between buds and bulbs. Nor need we wonder at the length of the umbilical cords of buds since that must correspond with their situation on the tree, in the same manner as their lymphatics and arteries are proportionally elongated. It does not appear probable that any umbilical artery attends these placental absorbents, since, as there seems to be no system of veins in vegetables to bring back the blood from the extremities of their arteries, (except their pulmonary veins,) there could not be any vegetable fluids to be returned to their placenta, which in vegetables seems to be simply an organ for nutrition, whereas the placenta of the animal foetus seems likewise to serve as a respiratory organ like the gills of fishes. NOTE XXXVI--VEGETABLE CIRCULATION. _And refluent blood in milky eddies bends._ CANTO IV. l. 420.