METHODS OF INSTRUCTION AND STUDY. At Penikese books were discarded, except for occasional reference, and the several students were encouraged to study the objects for which they had opportunity or special inclination. Each was also encouraged to select for himself some particular line of investigation and devote himself to that alone, and to a great extent this was done. There were the botanists, who gave special attention to the seaweeds and other plants; the ornithologists, who did nothing but collect, dissect, and preserve specimens of birds; the microscopists, who studied the diatoms and foraminifera and other microscopic forms, and made microscopic sections of tissues. One gave his entire attention to radiate animals, another to mollusks, and still another gave special attention to the development of the eggs of the tern. In the study of animals, about the first lesson impressed was the necessity of mastering their structure by the free use of the dissecting knife. For some days the toads of the island furnished specimens for the dissecting table; a day or two was devoted to the study of the internal as well as external structure of the lobster, each student dissecting one or more specimens. Then sharks, skates, star-fish, jelly-fish, flounders, sea anemones, turtles, birds, and even insects went under review in the same careful manner. A second lesson urged was the importance of studying the development of animals while in the egg or embryo state. Döllinger, the father of the now noted Dr. Döllinger, and in whose house Agassiz lived for four years, was the founder of this branch of scientific research, and gave Agassiz his first lessons in Embryology. Agassiz often alluded to him in terms of most tender recollection and regard. This man commenced his investigations in 1817, by opening three thousand eggs in various stages of development. Prof. Agassiz, while preparing the drawings and material for his study of the embryology of the turtle, published in the second volume of his “Contributions to the Natural History of America,” opened and examined many thousand turtles’ eggs. He frequently and earnestly urged attention to this, as an almost uncultivated field of research, and yet of vast importance in the solution of problems.