The several treaties concerning the western boundary of white settlement, concluded in close succession by North Carolina, Virginia, and the Crown with the Southern and Northern Indians, had an important bearing upon the settlement of Watauga. The Cherokee boundary line, as fixed by Governor Tryon (1767) and by John Stuart (1768), ran from Reedy River to Tryon Mountain, thence straight to Chiswell's Mine, and thence direct to the mouth of the Great Kanawha River. By the treaty at Fort Stanwix (November 5, 1768), in the negotiation of which Virginia was represented by Dr. Thomas Walker and Major Andrew Lewis, the Six Nations sold to the Crown their shadowy claim to a vast tract of western country, including in particular all the land between the Ohio and the Tennessee Rivers. The news of the cession resulted in a strong southwestward thrust of population, from the neighborhood of Abingdon, in the direction of the Holston Valley. [125] Recognizing that hundreds of these settlers were beyond the line negotiated by Stuart, but on lands not yet surveyed, Governor Botetourt instructed the Virginia commissioners to press for further negotiations, through Stuart, with the Cherokees. Accordingly, on October 18, 1770, a new treaty was made at Lochaber, South Carolina, by which a new line back of Virginia was established, beginning at the intersection of the North Carolina-Cherokee line (a point some seventy-odd miles east of Long Island), running thence in a west course to a point six miles east of Long Island, and thence in a direct course to the confluence of the Great Kanawha and Ohio Rivers. At the time of the treaty, it was agreed that the Holston River, from its intersection with the North Carolina-Virginia line, and down the course of the same, should be a temporary southern boundary of Virginia until the line should be ascertained by actual survey. [126] A strong influx of population into the immense new triangle thus released for settlement brought powerful pressure to bear upon northern Tennessee, the point of least resistance along the western barrier. Singularly enough, this advance was not opposed by the Cherokees, whose towns were strung across the extreme southeast corner of Tennessee.