Thus the body of the crayfish is resolvable, in the first place, into a repetition of similar segments, the _metameres_, each of which consists of a somite and two appendages; the metameres are built up out of a few simple _tissues_; and, finally, the tissues are either aggregates of more or less modified nucleated _cells_, or are products of such cells. Hence, in ultimate morphological analysis, the crayfish is a multiple of the histological unit, the nucleated cell. What is true of the crayfish, is certainly true of all animals, above the very lowest. And it cannot yet be considered certain that the generalization fails to hold good even of the simplest manifestations of animal life; since recent investigations have demonstrated the presence of a nucleus in organisms in which it had hitherto appeared to be absent. However this may be, there is no doubt that in the case of man and of all vertebrated animals, in that of all arthropods, mollusks, echinoderms, worms, and inferior organisms down to the very lowest sponges, the process of morphological analysis yields the same result as in the case of the crayfish. The body is built up of tissues, and the tissues are either obviously composed of nucleated cells; or, from the presence of nuclei, they may be assumed to be the results of the metamorphosis of such cells; or they are cuticular structures. The essential character of the nucleated cell is that it consists of a protoplasmic substance, one part of which differs somewhat in its physical and chemical characters {200} from the rest, and constitutes the nucleus. What part the nucleus plays in relation to the functions, or vital activities, of the cell is as yet unknown; but that it is the seat of operations of a different character from those which go on in the body of the cell is clear enough. For, as we have seen, however different the several tissues may be, the nuclei which they contain are very much alike; whence it follows, that if all these tissues were primitively composed of simple nucleated cells, it must be the bodies of the cells which have undergone metamorphosis, while the nuclei have remained relatively unchanged. On the other hand, when cells multiply, as they do in all growing parts, by the division of one cell into two, the signs of the process of internal change which ends in fission are apparent in the nucleus before they are manifest in the body of the cell; and, commonly, the division of the former precedes that of the latter. Thus a single cell body may possess two nuclei, and may become divided into two cells by the subsequent aggregation of the two moieties of its protoplasmic substance round each of them, as a centre.