The Effects of Cross & Self-Fertilisation in the Vegetable KingdomDarwin, Charles
Science
The Effects of Cross & Self-Fertilisation in the Vegetable Kingdom
Darwin, Charles
Fertilization of plants; Plants -- Variation; Pollination
Twelve flowers were crossed with pollen from a distinct plant, but
produced only six capsules, containing on an average 18.3 seeds.
Eighteen flowers were fertilised with their own pollen and produced ten
capsules, containing on an average 12.7 seeds, so that the seeds per
capsule were as 100 to 69. (5/25. Several species of Polemoniaceae are
known to be proterandrous, but I did not attend to this point in
Nemophila. Verlot says ‘Des Variétés’ 1865 page 66, that varieties
growing near one another spontaneously intercross.) The crossed seeds
weighed a little less than an equal number of self-fertilised seeds, in
the proportion of 100 to 105; but this was clearly due to some of the
self-fertilised capsules containing very few seeds, and these were much
bulkier than the others, from having been better nourished. A subsequent
comparison of the number of seeds in a few capsules did not show so
great a superiority on the side of the crossed capsules as in the
present case.
The seeds were placed on sand, and after germinating were planted in
pairs on the opposite sides of five pots, which were kept in the
greenhouse. When the seedlings were from 2 to 3 inches in height, most
of the crossed had a slight advantage over the self-fertilised. The
plants were trained up sticks, and thus grew to a considerable height.
In four out of the five pots a crossed plant flowered before any one of
the self-fertilised. The plants were first measured to the tips of their
leaves, before they had flowered and when the crossed were under a foot
in height. The twelve crossed plants averaged 11.1 inches in height,
whilst the twelve self-fertilised were less than half of this height,
namely, 5.45; or as 100 to 49. Before the plants had grown to their full
height, two of the self-fertilised died, and as I feared that this might
happen with others, they were again measured to the tops of their stems,
as shown in Table 5/72.
TABLE 5/72. Nemophila insignis; 0 means that the plant died.
Heights of plants measured in inches.
Column 1: Number (Name) of Pot.
Column 2: Crossed Plants.
Column 3: Self-fertilised Plants.
Pot 1 : 32 4/8 : 21 2/8.
Pot 2 : 34 4/8 : 23 5/8.
Pot 3 : 33 1/8 : 19.
Pot 3 : 22 2/8 : 7 2/8.
Pot 3 : 29 : 17 4/8.
Pot 4 : 35 4/8 : 10 4/8.
Pot 4 : 33 4/8 : 27.
Pot 5 : 35 : 0.
Pot 5 : 38 : 18 3/8.
Pot 5 : 36 : 20 4/8.
Pot 5 : 37 4/8 : 34.
Pot 5 : 32 4/8 : 0.
Total : 399.38 : 199.00.
The twelve crossed plants now averaged 33.28, and the ten
self-fertilised 19.9 inches in height, or as 100 to 60; so that they
differed somewhat less than before.
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