6. Pass the platinum loop into the tube containing the culture--do not allow the loop to touch the sides of the tube, or the handle to touch the medium--and remove a small portion of the growth; withdraw the loop from the tube, keeping the infected side of the loop downward. 7. Pass the loop into the bouillon tube almost down to the level of the fluid, reverse the loop so that the infected side faces upward, emulsify the portion of the growth in the moisture adhering to the side of the tube which is uppermost. Withdraw the loop. 8. Replug both tubes. 9. Sterilise the platinum loop. 10. Label the bouillon tube with (a) the name of the organism and (b) the date of inoculation. 11. Incubate. ~2. Solid Media.~--Solid media are stored in tubes in one of two ways: 1. Oblique tube or slanted tube (Fig. 118), in which the medium has been allowed to solidify whilst the tube was retained in an inclined position, so forming an extensive surface of medium extending from the bottom of the tube almost to its mouth. This is employed for "streak" or "smear" cultivations (_Strichcultur_). 2. Straight tube (Fig. 119), in which the medium forms a cylindrical mass in the lower portion of the tube and presents an upper surface which is at right angles to the long axis of the tube. This is employed for "stab" or "stick" cultivations (_Stichcultur_), or by inoculating the medium whilst fluid, and allowing to solidify in this position, for "shake" cultivations. _Streak Culture._-- 1. Flame the plugs, sterilise the platinum loop (or spatula). Open the tubes and charge the loop as in previous inoculation. 2. Pass the infected loop to the bottom of the tube to be inoculated and draw it, as lightly as possible, along the centre of the surface of the medium, terminating the "streak" over the thin layer of medium near the mouth of the tube. 3. Replug the tubes, sterilise the platinum loop. 4. Label the newly inoculated tube and incubate. _Smear Culture._--Proceed generally as in streak culture, but rub the infected loop all over the surface of the medium, instead of restricting the inoculation to a narrow line. NOTE.--Gelatine and agar oblique tubes should be freshly "slanted" before use. _Stab Culture._-- 1. Flame the plugs, open the tubes, sterilise the platinum needle and charge it with the inoculum as in the previous cultivations. 2. Pass the platinum needle into the tube to be inoculated until it touches the centre of the surface of the medium. Now thrust it deeply into the substance of the medium, keeping the needle as nearly as possible in the axis of the cylinder of medium. Then withdraw the needle. 3. Replug the tubes. Sterilise the platinum needle. 4. Label the newly planted tube and incubate.