The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.Eyre, J. W. H. (John William Henry)
Science
The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
Eyre, J. W. H. (John William Henry)
Bacteriology -- Technique
~Temperature.~--To take the rectal temperature of any of the laboratory
animals, the animal should be carefully and firmly held by an assistant.
Introduce the bulb of an ordinary clinical thermometer, well greased
with vaseline, just within the sphincter ani. Allow it to remain in this
position for a few seconds, and then push it on gently and steadily
until the entire bulb and part of the stem, as far as the constriction,
have passed into the rectum. Three to five minutes later, the time
varying of course with the sensibility of the thermometer used, withdraw
the instrument and take the reading. The thermometers employed for
recording temperature should be verified from time to time by comparison
with a standard Kew certified Thermometer kept in the laboratory for
that purpose.
[Illustration: FIG. 167.--Mouse scales]
~Cages.~--During the period which elapses between inoculation and death,
or complete recovery, the experimental animals must be kept in suitable
receptacles which can easily be kept clean and readily disinfected.
The _mouse_ is usually stored in a glass jar (Fig. 168) 11 cm. high and
11 cm. in diameter, closed by a wire gauze cover which is weighted with
lead or fastened to the mouth of the jar by a bayonet catch. A small
oblong label, 5 cm. by 2.5 cm., sand-blasted on the side of the
cylinder, is a very convenient device as notes made upon this with an
ordinary lead pencil show up well and only require the use of a damp
cloth to remove them (Fig. 168).
The _rat_ is kept under observation in a glass jar similar, but larger,
to that used for the mouse.
[Illustration: FIG. 168.--Mouse jar.]
[Illustration: FIG. 169.--Tripod.]
A layer of sawdust at the bottom of the jar absorbs any moisture and
cotton-wool or paper shavings should be provided for bedding. The food
should consist of bran and oats with an occasional feed of
bread-and-milk sop.
The use of a metal tripod, on the platform of which are soldered two
small cups for the reception of the food, inside the cage, prevents
waste of food or its contamination with excreta (Fig. 169).
After use the jars and tripods are sterilised either by chemical
reagents or by autoclaving.
The _rabbit_ and the _guinea-pig_ are confined in cages of suitable
size, made entirely of metal (Fig. 170). The sides and top and bottom
are of woven wire work; beneath the cage is a movable metal tray filled
with sawdust, for the reception of the excreta. The cage as a whole is
raised from the ground on short legs. The sides, etc., are generally
hinged so that the cage packs up flat, for convenience of storing and
also of sterilising.
The ordinary rat cage, a rectangular wire-work box, 30 cm. from front to
back, 20 cm. wide, and 14 cm. high, makes an excellent cage for
guinea-pigs if fitted with a shallow zinc tray, 35 cm. by 24 cm., for it
to stand upon.
[Illustration: FIG. 170.--Metal rabbit rage.]
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