The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.Eyre, J. W. H. (John William Henry)
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The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
Eyre, J. W. H. (John William Henry)
Bacteriology -- Technique
~Operation Table.~--This is a table of the "aseptic" type, composed of
steel tubing, nickel-plated or enamelled. The table-top frame is
sufficiently large to accommodate rabbits, dogs and monkeys; and is
supported upon telescopic uprights, so that it is adjustable as to
height; in its long axis it can be inclined (at either end) to 45° from
the horizontal. Further it can be completely rotated about its long
axis. The table-top itself is composed of a sheet of copper wire gauze
loosely suspended from the long sides of the tubular frame. The
slackness of the gauze bed permits of an india rubber hot water bottle,
or an electrotherm being placed under the animal, and if during the
course of an experiment it is necessary to reverse the animal, the
table-top frame is completely rotated, the device adopted for suspending
the gauze is detached and the gauze reversed also, so that it again
supports the animal from below.
[Illustration: FIG. 180.--Author's operating table[12].]
METHODS OF INOCULATION.
The following methods of inoculation apply more particularly to the
rabbit, but from them it will readily be seen what modifications in
technique, if any, are necessary in the case of the other experimental
animals.
~1. Cutaneous Inoculation.~--(_Anæsthetic, none._)
1. Have the animal firmly held by an assistant (or secured to the
operating table).
2. Apply the liquid soap to the fur, over the area selected for
inoculation, with a wad of cotton-wool, and lather freely by the aid of
warm water; shave carefully and thoroughly; or apply the depilatory
powder.
3. Wash the denuded area of skin thoroughly with 2 per cent. lysol
solution.
4. Wash off the lysol with ether and allow the latter to evaporate.
5. Make numerous short, parallel, superficial incisions with the point
of a sterile scalpel.
6. When the oozing from the incisions has ceased, rub the inoculum into
the scarifications by means of the flat of a scalpel blade, or a sterile
platinum spatula.
7. Cover the inoculated area with a pad of sterile gauze secured _in
situ_ by strips of adhesive plaster or by sealing down the edges of the
gauze with collodion.
8. Release the animal, place it in its cage, and affix a label upon
which is written:
(a) Distinctive name or number of the animal.
(b) Its weight.
(c) Particulars as to source and dose of inoculum.
(d) Date of inoculation.
~2. Subcutaneous Inoculation.~--
(a) _Fluid Inoculum._--(_Anæsthetic, none._)
Steps 1-4. As for cutaneous inoculation.
5. Pinch up a fold of skin between the forefinger and thumb of the left
hand; take the charged hypodermic syringe in the right hand, enter the
needle into a ridge of skin raised by the left finger and thumb, and
push it steadily onward until about 2 cm. of the needle are lying in the
subcutaneous tissue. Now release the grasp of the left hand and slowly
inject the fluid contained in the syringe.
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