2. _The weight_ of the inoculated animal should be observed and recorded each day during the course of an experimental infection at precisely the same hour, preferably just before the morning feed. 3. _The temperature_ should similarly be recorded daily, if not more frequently, during the whole period the animal is under observation, and carefully charted--individual variations will at once become apparent. It should be borne in mind that the temperature regarded as normal for man (37.5° C.) is not the normal average temperature of any of the lower animals save the rat and mouse. The accompanying table of normal averages for the animals usually employed in bacteriological research may be of use in preventing the erroneous assumption that pyrexia is present in an animal, which merely shows its own normal temperature. NORMAL AVERAGES. ---------------------------------------------------- | Rectal | Pulse. | Respirations. Animal. | Temp. °C.|------------------------ | | Rate per minute. ---------------------------------------------------- | | | Frog | 8.9-17.2 | 80 | 12 Mouse | 37.4 | 120 | ... Rat | 37.5 | ... | 210 Guinea pig | 38.6 | 150 | 80 Rabbit | 38.7 | 135 | 55 Cat | 38.7 | 130 | 24 Dog | 38.6 | 95 | 15 Goat | 40.0 | 75 | 16 Ox | 38.8 | 45 | .. Horse | 37.9 | 38 | 11 Monkey (Rhesus) | 38.4 | 100 | 19 Pigeon | 40.9 | 136 | 30 Fowl | 41.6 | 140 | 12 | | | ---------------------------------------------------- B. ~Special observations~ comprise some or all of the following, according to the method of inoculation and the character of the virus. 1. _The site of inoculation_ should be minutely examined at least at weekly intervals, and the neighbouring lymphatic glands palpated. 2. Any _local reaction_ at the site of inoculation and any other readily accessible lesion should be carefully investigated. Any suppurative process which may occur, whether in the subcutaneous tissues or in joints, should be explored and the pus carefully examined both microscopically and culturally. Fluid secretions and excretions, such as pus or serous exudates when accessible are collected direct from the body in sterile capillary pipettes (_vide_ Fig. 13a,) in the following manner: 1. Open the case containing the pipettes, grasp one by the plugged end, remove it from the case, and replace the lid of the latter. 2. Attach a rubber teat (_vide_ page 10) to the plugged end of the pipette and use the teat as the handle of the pipette. 3. Pass the entire length of the pipette twice or thrice through the flame of the Bunsen burner.