The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.Eyre, J. W. H. (John William Henry)
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The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
Eyre, J. W. H. (John William Henry)
Bacteriology -- Technique
limits.| millions.| thou-| 101)| | | 68) | |
| |sands.| | | | | |
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The above table represents in each case the average of a large number of
counts.
REMARKS.
_Frog._--The _red cells_ are large oval nucleated (20-25µ by 12-15µ)
discs, the nucleus relatively small and irregularly elongated or oval,
about 10µ in length. Many primitive and developing forms are usually
observed--also free nuclei and many cells in various stages of
degeneration. Hæmoglobin estimation is difficult owing to turbidity of
the blood after dilution with water. The _polymorphonuclear_ leucocytes
are large cells, about 20µ; no definite granules can be observed. The
_eosinophile_ cells contain large deeply staining coccal-shaped
granules.
_Mouse._--The granules of the _polymorphonuclear_ leucocytes are usually
not stained, or only very faintly so. The nucleus of the _eosinophile
cell_ is ring-shaped or much divided, and the granules are coccal and
stain oxyphile. The remarkable character of the blood is the high
percentage of large _mononuclear_ cells.
_Rat._--The fine rod-shaped granules of the _polymorphonuclear_
leucocytes are usually very faintly stained. The granules of
_eosinophile_ cells are well stained and coccal-shaped, the nucleus is
often ring shaped. The _basophile_ granular cells are few--but the
granules are large, and stain deeply basophile.
_Guinea-pig._--Polychromasia and punctate basophilia of _red cells_ are
very commonly observed--nucleated red cells are also frequent. The large
_mononuclear_ cells often contain vacuoles--"Kurlow cells"--possibly of
a parasitic nature.
_Rabbit._--It is not uncommon to find nucleated _red cells_ in films
from quite healthy animals. The granules of the _polymorphonuclear_
leucocytes stain oxyphile. The coarse granules of the _eosinophile_
cells appear to stain less deeply oxyphile, probably owing to the
basophile staining of the cytoplasm.
_Rhesus monkey._--The blood cells resemble those met with in human
blood. The minute neutrophile granules of the _polymorphonuclear_
leucocytes are often very scanty, and sometimes apparently absent. The
_eosinophile_ cells are not so densely packed with coarse oxpyhile
granules as in the human eosinophile, and the nuclei of these cells are
usually much divided, or polymorphous.
_Goat._--The _red cells_ are small, nonnucleated discs, only about 4.5µ
diameter, not much more than half that of the human red cell. The
_polymorphonuclear_ leucocytes have only a few very minute
coccal-shaped oxyphile granules, the nucleus is polymorphous. The
_eosinophile_ cells are large cells up to 20µ, the cytoplasm is
basophile and contains coarse coccal-shaped oxyphile granules, and the
nucleus is often much divided.
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