The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.Eyre, J. W. H. (John William Henry)
Science
The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
Eyre, J. W. H. (John William Henry)
Bacteriology -- Technique
_I. Tissues._--The naked-eye appearances of morbid tissues may be
preserved by the following method:
1. Remove the tissue or organ from the cadaver as soon after death as
possible, using great care to avoid distortion or injury.
2. Place it in a wide-mouthed stoppered jar, large enough to hold it
conveniently, resting on a pad of cotton-wool, and arrange it in the
position it is intended to occupy (but if it is intended to show a
section of the tissue or organ, do not incise it yet).
3. Cover with the Kaiserling fixing solution, and stopper the jar; allow
the tissues to remain in this solution for from forty-eight hours to
seven days (according to size) to fix. Make any necessary sections.
Kaiserling modified solution is prepared as follows:
Weigh out
Potassium acetate 30 grammes.
Potassium nitrate 15 grammes.
and dissolve in
Distilled water 1000 c.c.
then add
Formalin 150 c.c.
Filter.
This fixing solution can be used repeatedly so long as it remains clear.
Even when it has become turbid, if simple filtration is sufficient to
render it clear, the filtrate may be used again.
4. Transfer the tissue to a bath of methylated spirit (95 per cent.) for
thirty minutes to one hour.
5. Remove to a fresh bath of spirit and watch carefully. When the
natural colours show in their original tints, average time three to six
hours, remove the tissues from the spirit bath, dry off the spirit from
the cut surfaces by mopping with a soft cloth, then transfer to the
mounting solution.
Jore's mounting solution (modified) consists of
Glycerine 500 c.c.
Distilled water 750 c.c.
Formalin 2 c.c.
Equally good but much cheaper is Frost's mounting solution:
Potassium acetate 160 grammes.
Sodium fluoride 80 grammes.
Chloral hydrate 80 grammes.
Cane sugar (Tate's cubes) 3,500 grammes.
Saturated thymol water 8,000 c.c.
6. After twenty-four hours in this solution, or as soon as the tissue
sinks, transfer to a museum jar, fill with fresh mounting solution, and
seal.
_6a._ Or transfer to museum jar and fill with liquefied gelatine, to
which has been added 1 per cent. formalin. Cover the jar and allow the
gelatine to set. When solid, seal the cover of the jar in place.
7. To seal the museum preparation first warm the glass plate which forms
the cover. This is most conveniently done by placing the cleaned and
polished cover-plate upon a piece of asbestos millboard over a bunsen
flame turned low.
8. Smear an even layer of hot cement over the flange of the jar. The
cement is prepared as follows:
Weigh out and mix in an iron ladle
Gutta percha (pure) 4 parts.
Asphaltum 5 parts.
and melt together over a bunsen flame, stirring with an iron rod until
solution is complete.
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