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The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.Eyre, J. W. H. (John William Henry)

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The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.

Name: The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
Author: Eyre, J. W. H. (John William Henry)

Eyre, J. W. H. (John William Henry)

Bacteriology -- Technique

121°        |   1539.2     |         29.7        |     2.02
    122°        |   1588.4     |         30.7        |     2.09
    123°        |   1638.9     |         31.7        |     2.15
    124°        |   1690.7     |         32.7        |     2.22
    125°        |   1743.8     |         33.7        |     2.29
 ---------------+--------------+---------------------+-------------


TABLE FOR DESICCATION AT LOW TEMPERATURES IN VACUO.

+--------------------------+
| Temperature |            |
| Centigrade  | Mm. of Hg. |
+-------------+------------+
| 21°         | 18.4       |
| 22°         | 19.6       |
| 23°         | 20.8       |
| 24°         | 22.1       |
| 25°         | 23.5       |
|             |            |
| 26°         | 24.9       |
| 27°         | 26.4       |
| 28°         | 28.0       |
| 29°         | 29.7       |
| 30°         | 31.5       |
|             |            |
| 31°         | 33.3       |
| 32°         | 35.3       |
| 33°         | 37.3       |
| 34°         | 39.5       |
| 35°         | 41.7       |
|             |            |
| 36°         | 44.1       |
| 37°         | 46.6       |
| 38°         | 49.2       |
| 39°         | 51.9       |
| 40°         | 54.8       |
|             |            |
| 41°         | 57.8       |
| 42°         | 61.0       |
| 43°         | 64.3       |
| 44°         | 67.7       |
| 45°         | 71.3       |
|             |            |
| 46°         | 75.1       |
| 47°         | 79.0       |
| 48°         | 83.1       |
| 49°         | 87.4       |
| 50°         | 91.9       |
+-------------+------------+


ANTIFORMIN METHOD

For the detection of B. Tuberculosis.

_Antiformin_ was introduced into bacteriological technique by Uhlenhuth
in 1908 for the purpose of demonstrating tubercle bacilli when present
in small numbers, in sputum or other material. It is a powerful
oxidising agent and rapidly destroys most bacteria, but tubercle and
other acid-fast organisms resist its lethal action for considerable
periods, and upon this fact the method is based.

_To prepare Antiformin_ measure out and mix:--

Eau de Javelle (Liquor sodæ chlorinatæ--B.P.)  50 c.c.
Sodic hydrate 15 per cent. aqueous solution    50 c.c.

METHOD.

1. Introduce the sputum or other material (e. g. milk deposit and cream;
pus; minced gland or other organ; caseous material; broken down foci,
etc.) into a sterile tube and then add an equal volume of antiformin.

2. Close the tube with a rubber cork and shake vigorously (a sample of
antiformin that does not "foam" at this stage is of little use).
Disintegration of the material at once starts, associated bacteria are
destroyed and the mixture rapidly becomes a homogenous but turbid
fluid--a process which may be hastened by:--

3. Placing the tube in the incubator at 37° C. for 30 minutes--shaking
from time to time.

4. Centrifugalise the fluid thoroughly, at high speed.

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