Unequal total segmentation follows the same lines in the oldest fishes, the selachii and ganoids, which are directly descended from the cyclostoma. The primitive fishes (_Selachii_), which we must regard as the ancestral group of the true fishes, were generally considered, until a short time ago, to be discoblastic. It was not until the beginning of the twentieth century that Bashford Dean made the important discovery in Japan that one of the oldest living fishes of the shark type (_Cestracion japonicus_) has the same total unequal segmentation as the amphiblastic plated fishes (_ganoides_).[22] This is particularly interesting in connection with our subject, because the few remaining survivors of this division, which was so numerous in paleozoic times, exhibit three different types of gastrulation. [22] Bashford Dean, _Holoblastic Cleavage in the Egg of a Shark, Cestracion japonicus Macleay. Annotationes zoologicae japonenses,_ vol. iv, Tokio, 1901. Fig. 48 Sagittal section of the gastrula of the water-salamander. Fig. 48—Sagittal section of the gastrula of the water-salamander (_Triton_). (From _Hertwig._) Letters as in Fig. 47; except—_p_ yelk-stopper, _ mk_ beginning of the middle germinal layer.) The oldest and most conservative forms of the modern ganoids are the scaly sturgeons (Sturiones), plated fishes of great evolutionary importance, the eggs of which are eaten as caviar; their cleavage is not essentially different from that of the lampreys and the amphibia. On the other hand, the most modern of the plated fishes, the beautifully scaled bony pike of the North American rivers (Lepidosteus), approaches the osseous fishes, and is discoblastic like them. A third genus (Amia) is midway between the sturgeons and the latter. The group of the lung-fishes (_Dipneusta_ or _Dipnoi_) is closely connected with the older ganoids. In respect of their whole organisation they are midway between the gill-breathing fishes and the lung-breathing amphibia; they share with the former the shape of the body and limbs, and with the latter the form of the heart and lungs. Of the older dipnoi (_Paladipneusta_) we have now only one specimen, the remarkable Ceratodus of East Australia; its amphiblastic gastrulation has been recently explained by Richard Semon (cf. Chapter XXI). That of the two modern dipneusta, of which _ Protopterus_ is found in Africa and _Lepidosiren_ in America, is not materially different. (Cf. Fig. 51.) Fig. 49. Ovum-segmentation in the lamprey. Fig. 49—Ovum-segmentation of the lamprey (_Petromyzon fluviatalis_), in four successive stages. The small cells of the upper (animal) hemisphere divide much more quickly than the cells of the lower (vegetal) hemisphere.