In the meantime (1884) the studies of Wilhelm Haacke and Caldwell provided a proof of the long-suspected and very interesting fact, that the lowest mammals, the monotremes, _lay eggs,_ like the birds and reptiles, and are not viviparous like the other mammals. Although the gastrulation of the monotremes was not really known until studied by Richard Semon in 1894, there could be little doubt, in view of the great size of their food-yelk, that their ovum-segmentation was discoid, and led to the formation of a sickle-mouthed discogastrula, as in the case of the reptiles and birds. Hence I had, in 1875 (in my essay on _The Gastrula and Ovum-segmentation of Animals_), counted the monotremes among the discoblastic vertebrates. This hypothesis was established as a fact nineteen years afterwards by the careful observations of Semon; he gave in the second volume of his great work, _Zoological Journeys in Australia_ (1894), the first description and correct explanation of the discoid gastrulation of the monotremes. The fertilised ova of the two living monotremes (_Echidna_ and _ Ornithorhynchus_) are balls of one-fifth of an inch in diameter, enclosed in a stiff shell; but they grow considerably during development, so that when laid the egg is three times as large. The structure of the plentiful yelk, and especially the relation of the yellow and the white yelk, are just the same as in the reptiles and birds. As with these, partial cleavage takes place at a spot on the surface at which the small formative yelk and the nucleus it encloses are found. First is formed a lens-shaped circular germinal disk. This is made up of several strata of cells, but it spreads over the yelk-ball, and thus becomes a one-layered blastula. Fig.63. Ovum of the opossum (Didelphys) divided into four. Fig. 63—Ovum of the opossum (_Didelphys_) divided into four. (From _Selenka._) _b_ the four segmentation-cells, _r_ directive body, _c_ unnucleated coagulated matter, _p,_ albumin-membrane. If we then imagine the yelk it contains to be dissolved and replaced by a clear liquid, we have the characteristic blastula of the higher mammals. In these the gastrulation proceeds in two phases, as Semon rightly observes: firstly, formation of the entoderm by cleavage at the centre and further growth at the edge; secondly, invagination. In the monotremes more primitive conditions have been retained better than in the reptiles and birds. In the latter, before the commencement of the gastrula-folding, we have, at least at the periphery, a two-layered embryo forming from the cleavage. But in the monotremes the formation of the cenogenetic entoderm does not precede the invagination; hence in this case the construction of the germinal layers is less modified than in the other amniota.