Cœlomation takes place with equal clearness and transparency in the case of the amphioxus, the lowest vertebrate, and its nearest relatives, the invertebrate tunicates, the sea-squirts. However, in these two stems, which we class together as _ Chordonia,_ this important process is more complex, as two other processes are associated with it—the development of the chorda from the entoderm and the separation of the medullary plate or nervous centre from the ectoderm. Here again the skulless amphioxus has preserved to our own time by tenacious heredity the chief phenomena in their original form, while it has been more or less modified by embryonic adaptation in all the other vertebrates (with skulls). Hence we must once more thoroughly understand the palingenetic embryonic features of the lancelet before we go on to consider the cenogenetic forms of the craniota. Figs. 81 and 82. Transverse section of amphioxus embryo. Figs. 81 and 82.—Transverse section of amphioxus embryo. Fig. 81 at the stage with five somites, Fig. 82 at the stage with eleven somites. (From _Hatschek._) _ak_ outer germinal layer, _mp_ medullary plate, _n_ nerve-tube, _ik_ inner germinal layer, _dh_ visceral cavity, _lh_ body-cavity, _mk_ middle germinal layer (_mk_1 parietal, _mk_2 visceral), _us_ primitive segment, _ ch_ chorda. The cœlomation of the amphioxus, which was first observed by Kowalevsky in 1867, has been very carefully studied since by Hatschek (1881). According to him, there are first formed on the bilateral gastrula we have already considered (Figs. 36, 37) three parallel longitudinal folds—one single ectodermal fold in the central line of the dorsal surface, and a pair of entodermic folds at the two sides of the former. The broad ectodermal fold that first appears in the middle line of the flattened dorsal surface, and forms a shallow longitudinal groove, is the beginning of the central nervous system, the medullary tube. Thus the primary outer germinal layer divides into two parts, the middle medullary plate (Fig. 81 _mp_) and the horny-plate (_ak_), the beginning of the outer skin or epidermis. As the parallel borders of the concave medullary plate fold towards each other and grow underneath the horny-plate, a cylindrical tube is formed, the medullary tube (Fig. 82 _n_); this quickly detaches itself altogether from the horny-plate. At each side of the medullary tube, between it and the alimentary tube (Figs. 79–82 _dh_), the two parallel longitudinal folds grow out of the dorsal wall of the alimentary tube, and these form the two cœlom-pouches (Figs. 80, 81 _ lh_). This part of the entoderm, which thus represents the first structure of the middle germinal layer, is shown darker than the rest of the inner germinal layer in Figs. 79–82. The edges of the folds meet, and thus form closed tubes (Fig. 81 in section).