We may now disregard both the outer ovolemma and the greater part of the vesicle, and concentrate our attention on the germinative area and the four-layered embryonic disk. It is here alone that we find the important changes which lead to the differentiation of the first organs. It is immaterial whether we examine the germinative area of the mammal (the rabbit, for instance) or the germinal disk of a bird or a reptile (such as a lizard or tortoise). The embryonic processes we are now going to consider are essentially the same in all members of the three higher classes of vertebrates which we call the amniotes. Man is found to agree in this respect with the rabbit, dog, ox, etc.; and in all these animals the germinative area undergoes essentially the same changes as in the birds and reptiles. They are most frequently and accurately studied in the chick, because we can have incubated hens' eggs in any quantity at any stage of development. Moreover, the round germinal disk of the chick passes immediately after the beginning of incubation (within a few hours) from the two-layered to the four-layered stage, the two-layered mesoderm developing from the median primitive groove between the ectoderm and entoderm (Figures 1.82 to 1.95). The first change in the round germinal disk of the chick is that the cells at its edges multiply more briskly, and form darker nuclei in their protoplasm. This gives rise to a dark ring, more or less sharply set off from the lighter centre of the germinal disk (Figure 1.115). From this point the latter takes the name of the "light area" (area pellucida), and the darker ring is called the "dark area" (area opaca). (In a strong light, as in Figures 1.115 to 1.117, the light area seems dark, because the dark ground is seen through it; and the dark area seems whiter). The circular shape of the area now changes into elliptic, and then immediately into oval (Figures 1.116 and 1.117). One end seems to be broader and blunter, the other narrower and more pointed; the former corresponds to the anterior and the latter to the posterior section of the subsequent body. At the same time, we can already trace the characteristic bilateral form of the body, the antithesis of right and left, before and behind. This will be made clearer by the "primitive streak," which appears at the posterior end. (FIGURE 1.118. Pear-shaped germinal shield of the rabbit (eight days old), magnified twenty times. rf medullary groove. pr primitive groove (primitive mouth). (From Kolliker.) FIGURE 1.119. Median longitudinal section of the gastrula of four vertebrates. (From Rabl.) A discogastrula of a shark (Pristiurus). B amphigastrula of a sturgeon (Accipenser). C amphigastrula of an amphibium (Triton). D epigastrula of an amniote (diagram). a ventral, b dorsal lip of the primitive mouth.)