The little algæ on their part find a peaceful and relatively secure
abode within the polyp, and they apparently do not occur outside of
it, at least they do not now migrate from outside into the animal,
but are carried over as a heritable possession of the polyps from
one generation to another, and in a very interesting manner, namely,
by means of the eggs, and by these alone. As Hamann has shown, the
zoochlorellæ migrate at the time when an egg is formed in the outer
layer of the body of the polyp (Fig. 35) from the inner layer outwards,
piercing through the supporting layer between them (st) and penetrating
into the egg (_B_, _Eiz_). They make their way only into the egg, not
into the sperm-cells, which in any case are too small to include them.
Thus they are absent from no young polyp of this species, and it is
easy to understand why earlier experimental attempts to rear colourless
polyps from eggs could never succeed even in the purest water.
[Illustration: FIG. 36. _A_, _Amœba viridis_. _k_, the nucleus.
_cv_, contractile vacuole. _zchl_, the zoochlorellæ. _B_, a single
zoochlorella under high power. After A. Gruber.]
Quite similar green algæ live in symbiosis with unicellular animals,
as, for instance, with an amœba (Fig. 36) and with an Infusorian of the
genus _Bursaria_. In the Zoological Institute in Freiburg there is a
living colony of a green amœba and a green _Bursaria_, both of which
came from America, sent to us some years ago by Professor Wilder, of
Chicago, inside a letter with dried _Sphagnum_, or bog-moss. The plants
came from stagnant water in the Connecticut valley in Massachusetts.
That in this case the zoochlorellæ are of use to the animals within
which they live, not only by giving off oxygen, but also by yielding
food-stuff, has been proved by A. Gruber, who bred the two green
species for seven years in pure water which contained no trace of any
kind of organic food for them. Nevertheless, they multiplied rapidly,
and still form a green scum on the walls of the glass in which they are
kept. They only die away when they are kept in the dark, where the algæ
are unable to assimilate; then one green cell after another wanes and
disappears, and, in consequence, their hosts also die from the double
cause of lack of oxygen and lack of food.
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