The emperor also wrote to Margaret on the 15th January asking her to convoke the States-General for the 22nd of May, to inform them of the peace that had just been concluded. But Francis had no intention of keeping the promises which had been wrung from him under compulsion, and he secretly resolved to break faith with the emperor as soon as he regained his liberty. A few days after the Treaty of Madrid had been signed Margaret had the sorrow of losing her niece, Isabel, the young Queen of Denmark, who died near Ghent on the 19th of January, at the age of twenty-five, and was buried in that city. Her life with Christian II. had not been a happy one, and it was said that she died of a broken heart. Her three children, John, Dorothea, and Christina,[114] she left to her aunt Margaret's care, 'whom she had always called her mother.' Margaret nobly fulfilled this trust, and tenderly watched over the children until her death. She appointed the learned Cornelius Agrippa, then residing at her Court, as tutor to Prince John, who at the time of his mother's death was only eight years old. In a letter to Ferdinand Charles thus mentions their sister's death: 'I am very sorry for the death of our sister the Queen of Denmark, and have taken care that prayers should be said for the repose of her soul. I would willingly recommend to you her children our nephews, who are at present in the hands of our dear aunt in Flanders.' [114] Christina married first Francesco Sforza, Duke of Milan, and secondly the Duke of Lorraine. Her beautiful portrait by Holbein, lent by the Duke of Norfolk, hangs in the National Gallery. Her elder sister, Dorothea, married Frederick, Count Palatine. The portraits of Isabel's three children in one picture by Mabuse are at Hampton Court Palace. [Illustration: THE CHILDREN OF CHRISTIAN II AND ISABEL OF DENMARK IN MOURNING DRESS FOR THEIR MOTHER FROM THE PAINTING BY MABUSE AT HAMPTON COURT PALACE] On Ash-Wednesday, the 14th of February, Charles de Lannoy wrote to Margaret from Madrid to inform her that the emperor had arrived the day before, and King Francis had gone outside the city to meet him. After supper they had spent two hours talking together, and seemed well pleased with each other. The king had begged permission to see Queen Eleanor, which was granted, with the assurance that as soon as he set foot in Provence she should be delivered over to him. Lannoy goes on to say that he has been ordered to attend the king on his way to France.